EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.21.3.1 | ascorbic acid | - |
Aspergillus nidulans | |
1.21.3.1 | dithiothreitol | - |
Aspergillus nidulans | |
1.21.3.1 | O2 | required | Aspergillus nidulans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.21.3.1 | expression of wild-type and mutants as maltose-binding fusion proteins in Escherichia coli | Aspergillus nidulans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.21.3.1 | L223A | reduced activity with N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate is a poor substrate | Aspergillus nidulans |
1.21.3.1 | L223I | reduced activity with N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine, product spectrum differs from that of the wild-type with delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate as substrate | Aspergillus nidulans |
1.21.3.1 | L223V | reduced activity with N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine, product spectrum differs from that of the wild-type with delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate as substrate | Aspergillus nidulans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.21.3.1 | Fe2+ | - |
Aspergillus nidulans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Aspergillus nidulans | catalytic reaction is under steric regulation | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Aspergillus nidulans | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.21.3.1 | Aspergillus nidulans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.21.3.1 | recombinant wild-type and mutants from Escherichia coli | Aspergillus nidulans |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O | structure and mechanism | Aspergillus nidulans | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O | catalytic reaction is under steric regulation | Aspergillus nidulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.3.1 | delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-alpha-aminobutyrate + O2 | wild-type and mutants, reaction mechanism | Aspergillus nidulans | ? | - |
ir | |
1.21.3.1 | additional information | delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alpha-aminobutyrate as substrate is converted to 3 different products: an alpha- and a beta-methyl-penam, and a cepham | Aspergillus nidulans | ? | - |
? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | catalytic reaction is under steric regulation | Aspergillus nidulans | isopenicillin N + 2 H2O | - |
? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | catalytic reaction is under steric regulation | Aspergillus nidulans | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
1.21.3.1 | N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Aspergillus nidulans | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.21.3.1 | IPNS | - |
Aspergillus nidulans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.21.3.1 | 27 | - |
assay at | Aspergillus nidulans |