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Literature summary extracted from

  • Durairaj, M.; Leskiw, B.K.; Jensen, S.E.
    Genetic and biochemical analysis of the cysteinyl residues of isopenicillin N synthase from Streptomyces clavuligerus (1996), Can. J. Microbiol., 42, 870-875.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.3.1 ascorbic acid
-
Streptomyces clavuligerus
1.21.3.1 dithiothreitol
-
Streptomyces clavuligerus
1.21.3.1 O2 required Streptomyces clavuligerus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.1 expression of wild-type and mutants in Escherichia coli Streptomyces clavuligerus

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.1 C104S single-strand-site-directed mutagenesis, loss of more than 96% activity Streptomyces clavuligerus
1.21.3.1 C142S single-strand-site-directed mutagenesis, loss of 24% activity Streptomyces clavuligerus
1.21.3.1 C251S single-strand-site-directed mutagenesis, loss of 47.7% activity Streptomyces clavuligerus
1.21.3.1 C37S single-strand-site-directed mutagenesis, loss of 18.3% activity Streptomyces clavuligerus
1.21.3.1 C37S/C142S/C251S triple mutant, conformationally different from wild-type, prepared by recombining fragments of IPNS-encoding gene pcbC from each of the single mutants, loss of more than 90% activity Streptomyces clavuligerus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.3.1 N-ethylmaleimide wild-type and mutants, binds to Cys104 in the active site Streptomyces clavuligerus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.1 Fe2+ required Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Streptomyces clavuligerus common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O product has antibiotic activity ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Streptomyces clavuligerus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.1 partially, wild-type and mutants recombinant from Escherichia coli Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.3.1 0.0000000125
-
mutant C104S, recombinant, partially purified Streptomyces clavuligerus
1.21.3.1 0.000000177
-
mutant C251S, recombinant, partially purified Streptomyces clavuligerus
1.21.3.1 0.000000257
-
mutant C142S, recombinant, partially purified Streptomyces clavuligerus
1.21.3.1 0.000000276
-
mutant C37S, recombinant, partially purified Streptomyces clavuligerus
1.21.3.1 0.000000338
-
wild-type, recombinant, partially purified Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
Streptomyces clavuligerus isopenicillin N + 2 H2O
-
?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Streptomyces clavuligerus isopenicillin N + 2 H2O product has antibiotic activity ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
Streptomyces clavuligerus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.3.1 26
-
assay at Streptomyces clavuligerus