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Literature summary extracted from

  • Roach, P.L.; Clifton, I.J.; Fulop, V.; Harlos, K.; Barton, G.J.; Hajdu, J.; Andersson, I.; Schofield, C.J.; Baldwin, J.E.
    Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes (1995), Nature, 375, 700-704.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.3.1 O2 required Aspergillus nidulans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.21.3.1 enzyme complexed with manganese instead of iron in the active site, more stable Aspergillus nidulans
1.21.3.1 structure analysis Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.1 Fe2+
-
 Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Aspergillus nidulans common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O product has antibiotic activity ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Aspergillus nidulans P05326 accession number SwissProt
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O structure and mechanism Aspergillus nidulans
a
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O active site with conserved jelly-roll motif, cysteine residues are not directly involved in the coordination of the metal ion Aspergillus nidulans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Aspergillus nidulans isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Aspergillus nidulans isopenicillin N + 2 H2O product has antibiotic activity ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Aspergillus nidulans