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Literature summary extracted from

  • Tan, D.S.H.; Sim, T.S.
    Functional analysis of conserved histidine residues in Cephalosporium acremonium isopenicillin N synthase by site-directed mutagenesis (1996), J. Biol. Chem., 271, 889-894.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.3.1 O2 required Acremonium chrysogenum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.1 expression of wild-type and mutants in Escherichia coli, amino acid sequence comparison Acremonium chrysogenum

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.1 H116L site-directed mutagenesis, reduced activity Acremonium chrysogenum
1.21.3.1 H126L site-directed mutagenesis, reduced activity Acremonium chrysogenum
1.21.3.1 H137L site-directed mutagenesis, reduced activity Acremonium chrysogenum
1.21.3.1 H262L site-directed mutagenesis, complete loss of activity Acremonium chrysogenum
1.21.3.1 H272L site-directed mutagenesis, complete loss of activity Acremonium chrysogenum
1.21.3.1 H49L site-directed mutagenesis, complete loss of activity Acremonium chrysogenum
1.21.3.1 H64L site-directed mutagenesis, reduced activity Acremonium chrysogenum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.1 Fe2+ required Acremonium chrysogenum
1.21.3.1 Fe2+ His216 and His 272 are ligands to bind the non-heme iron in the active site Acremonium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.3.1 38000
-
 x * 38000, wild-type and mutants, SDS-PAGE Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Acremonium chrysogenum common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Acremonium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.1 wild-type and mutants recombinant from Escherichia coli Acremonium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.3.1 additional information
-
-
 Acremonium chrysogenum
1.21.3.1 0.00000224
-
 mutant H49L Acremonium chrysogenum
1.21.3.1 0.0000103
-
 mutant H126L Acremonium chrysogenum
1.21.3.1 0.0000112
-
 mutant H137L Acremonium chrysogenum
1.21.3.1 0.0000116
-
 mutant H116L Acremonium chrysogenum
1.21.3.1 0.0000127
-
 mutant H64L Acremonium chrysogenum
1.21.3.1 0.0000136
-
 wild-type Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Acremonium chrysogenum isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Acremonium chrysogenum isopenicillin N + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.3.1 ? x * 38000, wild-type and mutants, SDS-PAGE Acremonium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Acremonium chrysogenum