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Literature summary extracted from

  • Khurana, S.; Sanli, G.; Powers, D.B.; Anderson, S.; Blaber, M.
    Molecular modeling of substrate binding in wild-type and mutant Corynebacteria 2,5-diketo-D-gluconate reductases (2000), Proteins, 39, 68-75.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.274 F22Y isoenzyme A, similar kcat as wild-type Corynebacterium sp.
1.1.1.274 Q192R isoenzyme A, 2.5fold increase in kcat Corynebacterium sp.
1.1.1.346 F22Y the mutation causes a 2.5fold decrease in Km for 2,5-didehydro-D-gluconate whereas the value of kcat remains essentially unchanged Corynebacterium sp.
1.1.1.346 Q192R the mutation primarily affects the kcat parameter toward the 2,5-didehydro-D-gluconate substrate, increasing its value approximately 2.5fold, whereas Km is relatively unaffected, or increases slightly Corynebacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.274 0.0123
-
 2,5-didehydro-D-gluconate isoenzyme A, F22Y mutant enzyme Corynebacterium sp.
1.1.1.274 0.0312
-
 2,5-didehydro-D-gluconate isoenzyme A, wild-type Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.274 Corynebacterium sp.
-
 isoenzyme A
-
1.1.1.346 Corynebacterium sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.274 2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH + H+ reaction mechanism Corynebacterium sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.346 2,5-didehydro-D-gluconate + NADPH + H+ isoform DKGR B exhibits 66fold higher specific activity toward 2,5-didehydro-D-gluconate than isoform DKGR A Corynebacterium sp. 2-dehydro-L-gulonate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.346 2,5-diketo-D-gluconate reductase
-
 Corynebacterium sp.
1.1.1.346 2,5-DKGR A isoform Corynebacterium sp.
1.1.1.346 2,5-DKGR B isoform Corynebacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.346 NADPH dependent on Corynebacterium sp.