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Literature summary extracted from

  • Khurana, S.; Powers, D.B.; Anderson, S.; Blaber, M.
    Crystal structure of 2,5-diketo-D-gluconic acid reductase A complexed with NADPH at 2.1 A resolution (1998), Proc. Natl. Acad. Sci. USA, 95, 6768-6773.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.274 isoenzyme A, expression in Acetobacter cerinus Corynebacterium sp.
1.1.1.346 expressed in in Acetobacter cerinus strain IFO 3263 Corynebacterium sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.274 isoenzyme A, hanging-drop vapour diffusion, X-ray structure of complex with NADPH, 2.1 A resolution Corynebacterium sp.
1.1.1.346 in complex with NADPH, hanging drop vapor diffusion method, using 1 M sodium phosphate, 1 M potassium phosphate, 100 mM HEPES buffer, pH 7.0, at 4°C Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.274 Corynebacterium sp.
-
-
-
1.1.1.346 Corynebacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.274 recombinant isoenzyme A Corynebacterium sp.
1.1.1.346
-
 Corynebacterium sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.274 2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH + H+ catalytic mechanism Corynebacterium sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.346 2,5-didehydro-D-gluconate + NADPH + H+
-
 Corynebacterium sp. 2-dehydro-L-gulonate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.346 2,5-diketo-D-gluconic acid reductase A
-
 Corynebacterium sp.
1.1.1.346 2,5-DKGR A
-
 Corynebacterium sp.
1.1.1.346 AKR5C
-
 Corynebacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.346 NADPH
-
 Corynebacterium sp.