EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.274 | Cu2+ | 0.5 mM, strong inhibition | Corynebacterium sp. | |
1.1.1.274 | Fe3+ | 0.5 mM, strong inhibition | Corynebacterium sp. | |
1.1.1.274 | NADP+ | competitive product inhibition | Corynebacterium sp. | |
1.1.1.274 | Ni2+ | 0.5 mM, strong inhibition | Corynebacterium sp. | |
1.1.1.274 | Zn2+ | 0.5 mM, strong inhibition | Corynebacterium sp. | |
1.1.1.346 | Cu2+ | strong inhibition at 0.5 mM | Corynebacterium sp. | |
1.1.1.346 | Fe3+ | strong inhibition at 0.5 mM | Corynebacterium sp. | |
1.1.1.346 | additional information | not inhibited by 2-dehydro-L-gulonate. There is no observed effect on activity by 0.5 mM solutions of Mg2+, Mn2+, Ca2+, or Co2+, 14 mM 2-mercaptoethanol, 1 mM dithiothreitol, or 1 mM EDTA | Corynebacterium sp. | |
1.1.1.346 | NADP+ | competitive inhibition | Corynebacterium sp. | |
1.1.1.346 | Ni2+ | strong inhibition at 0.5 mM | Corynebacterium sp. | |
1.1.1.346 | Zn2+ | strong inhibition at 0.5 mM | Corynebacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.274 | 0.01 | - |
NADPH | - |
Corynebacterium sp. | |
1.1.1.274 | 2.2 | - |
glycerol | - |
Corynebacterium sp. | |
1.1.1.274 | 26 | - |
2,5-didehydro-D-gluconate | - |
Corynebacterium sp. | |
1.1.1.274 | 155 | - |
5-keto-D-fructose | - |
Corynebacterium sp. | |
1.1.1.274 | 160 | - |
dihydroxyacetone | - |
Corynebacterium sp. | |
1.1.1.346 | 0.01 | - |
NADPH | at pH 6.4 and 25°C | Corynebacterium sp. | |
1.1.1.346 | 0.125 | - |
NADP+ | at pH 9.2 and 25°C | Corynebacterium sp. | |
1.1.1.346 | 26 | - |
2,5-didehydro-D-gluconate | at pH 6.4 and 25°C | Corynebacterium sp. | |
1.1.1.346 | 155 | - |
5-dehydro-D-fructose | at pH 6.4 and 25°C | Corynebacterium sp. | |
1.1.1.346 | 160 | - |
dihydroxyacetone | at pH 6.4 and 25°C | Corynebacterium sp. | |
1.1.1.346 | 204 | - |
2-dehydro-L-gulonate | at pH 9.2 and 25°C | Corynebacterium sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.346 | cytosol | - |
Corynebacterium sp. | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.274 | 29992 | - |
1 * 29992, deduced from nucleotide sequence | Corynebacterium sp. |
1.1.1.274 | 34000 | - |
1 * 34000, SDS-PAGE | Corynebacterium sp. |
1.1.1.274 | 35000 | - |
gel filtration | Corynebacterium sp. |
1.1.1.346 | 34000 | - |
1 * 34000, SDS-PAGE | Corynebacterium sp. |
1.1.1.346 | 35000 | - |
gel filtration | Corynebacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.274 | Corynebacterium sp. | - |
- |
- |
1.1.1.346 | Corynebacterium sp. | - |
- |
- |
1.1.1.346 | Corynebacterium sp. ATCC 31090 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.274 | DEAE-cellulose, Cibacron blue F3GA agarose, gel filtration | Corynebacterium sp. |
1.1.1.346 | DEAE-cellulose column chromatography, Cibacron blueF 3GA affinity column chromatography, and and TSK gel filtration | Corynebacterium sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.274 | 3.38 | - |
- |
Corynebacterium sp. |
1.1.1.346 | 0.0052 | - |
crude extract, at pH 6.4 and 25°C | Corynebacterium sp. |
1.1.1.346 | 3.38 | - |
after 650fold purification, at pH 6.4 and 25°C | Corynebacterium sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.274 | -70°C, 20 mM Tris-HCl, pH 7.5, over 6 months, no loss of activity | Corynebacterium sp. |
1.1.1.274 | 4°C, pH 6.5-7.5, at least 2 months, no loss of activity | Corynebacterium sp. |
1.1.1.346 | -70°C, 2 mg/ml enzyme in 20 mM Tris-HC1 (pH 7.5), 6 months, the enzyme remains stable | Corynebacterium sp. |
1.1.1.346 | 4°C, enzyme solution at pH 6.5-7.5, at least 2 months, the enzyme remains stable | Corynebacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.274 | 2,5-didehydro-D-gluconate + NADH | 170fold lower reduction rate with NADH compared to NADPH | Corynebacterium sp. | 2-keto-L-gulonate + NAD+ | - |
? | |
1.1.1.274 | 2,5-didehydro-D-gluconate + NADH | 170fold lower reduction rate with NADH compared to NADPH | Corynebacterium sp. ATCC 31090 | 2-keto-L-gulonate + NAD+ | - |
? | |
1.1.1.274 | 2,5-didehydro-D-gluconate + NADPH | stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate | Corynebacterium sp. | 2-keto-L-gulonate + NADP+ | - |
r | |
1.1.1.274 | 2,5-didehydro-D-gluconate + NADPH | stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate | Corynebacterium sp. ATCC 31090 | 2-keto-L-gulonate + NADP+ | - |
r | |
1.1.1.274 | 5-keto-D-fructose + NADPH | - |
Corynebacterium sp. | L-sorbose + NADP+ | - |
? | |
1.1.1.274 | 5-keto-D-fructose + NADPH | - |
Corynebacterium sp. ATCC 31090 | L-sorbose + NADP+ | - |
? | |
1.1.1.274 | dihydroxyacetone + NADPH | - |
Corynebacterium sp. | glycerol + NADP+ | - |
? | |
1.1.1.274 | dihydroxyacetone + NADPH | - |
Corynebacterium sp. ATCC 31090 | glycerol + NADP+ | - |
? | |
1.1.1.346 | 2,5-didehydro-D-gluconate + NADPH + H+ | a reduction of substrate by NADPH is highly preferred | Corynebacterium sp. | 2-dehydro-L-gulonate + NADP+ | - |
r | |
1.1.1.346 | 2,5-didehydro-D-gluconate + NADPH + H+ | a reduction of substrate by NADPH is highly preferred | Corynebacterium sp. ATCC 31090 | 2-dehydro-L-gulonate + NADP+ | - |
r | |
1.1.1.346 | 2-dehydro-L-gulonate + NADP+ | - |
Corynebacterium sp. | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
r | |
1.1.1.346 | 5-dehydro-D-fructose + NADPH + H+ | - |
Corynebacterium sp. | ? + NADP+ | - |
? | |
1.1.1.346 | dihydroxyacetone + NADPH + H+ | - |
Corynebacterium sp. | ? + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.274 | monomer | 1 * 34000, SDS-PAGE | Corynebacterium sp. |
1.1.1.274 | monomer | 1 * 29992, deduced from nucleotide sequence | Corynebacterium sp. |
1.1.1.346 | monomer | 1 * 34000, SDS-PAGE | Corynebacterium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.346 | 2,5-diketo-D-gluconate reductase | - |
Corynebacterium sp. |
1.1.1.346 | 2,5-DKG reductase | - |
Corynebacterium sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.274 | 8.33 | - |
2,5-didehydro-D-gluconate | - |
Corynebacterium sp. | |
1.1.1.346 | 8.3 | - |
2,5-didehydro-D-gluconate | at pH 6.4 and 25°C | Corynebacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.274 | 6.4 | - |
reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
1.1.1.274 | 9.2 | - |
oxidation of 2-keto-L-gulonate | Corynebacterium sp. |
1.1.1.346 | 6.4 | - |
for the reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
1.1.1.346 | 9.2 | - |
for the oxidation of 2-dehydro-L-gulonate | Corynebacterium sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.274 | 4.5 | 9.3 | reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
1.1.1.274 | 8 | 10.3 | oxidation of 2-keto-L-gulonate | Corynebacterium sp. |
1.1.1.346 | 5 | 8 | for the reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.274 | NADP+ | - |
Corynebacterium sp. | |
1.1.1.274 | NADPH | - |
Corynebacterium sp. | |
1.1.1.346 | NADPH | dependent on | Corynebacterium sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.274 | 0.026 | - |
NADP+ | - |
Corynebacterium sp. | |
1.1.1.346 | 0.026 | - |
NADP+ | at pH 9.2 and 25°C | Corynebacterium sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.346 | Corynebacterium sp. | isoelectric focusing | - |
4.4 |