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Literature summary extracted from

  • Sonoyama, T.; Kobayashi, K.
    Purification and properties of two 2,5-diketo-D-gluconate reductases from a mutant strain derived from Corynebacterium sp (1987), J. Ferment. Technol., 65, 311-317.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.274 1.8
-
 2,5-didehydro-D-gluconate isoenzyme I Corynebacterium sp.
1.1.1.274 12
-
 NADPH isoenzyme I Corynebacterium sp.
1.1.1.274 13
-
 NADPH isoenzyme II Corynebacterium sp.
1.1.1.274 13.5
-
 2,5-didehydro-D-gluconate isoenzyme II Corynebacterium sp.
1.1.1.346 0.012
-
 NADPH isoform 2,5-diketo-D-gluconate reductase I, at pH 7.0 and 30°C Corynebacterium sp.
1.1.1.346 0.013
-
 NADPH isoform 2,5-diketo-D-gluconate reductase II, at pH 7.0 and 30°C Corynebacterium sp.
1.1.1.346 1.8
-
 2,5-didehydro-D-gluconate isoform 2,5-diketo-D-gluconate reductase I, at pH 7.0 and 30°C Corynebacterium sp.
1.1.1.346 13.5
-
 2,5-didehydro-D-gluconate isoform 2,5-diketo-D-gluconate reductase II, at pH 7.0 and 30°C Corynebacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.274 29000
-
 isoenzyme I Corynebacterium sp.
1.1.1.274 34000
-
 isoenzyme II Corynebacterium sp.
1.1.1.346 29000
-
 1 * 29000, isoform 2,5-diketo-D-gluconate reductase I, SDS-PAGE Corynebacterium sp.
1.1.1.346 34000
-
 1 * 34000, isoform 2,5-diketo-D-gluconate reductase II, SDS-PAGE Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.274 Corynebacterium sp.
-
 2,5-diketo-D-gluconate reductases I and II
-
1.1.1.346 Corynebacterium sp.
-
-
-
1.1.1.346 Corynebacterium sp. SHS 0007
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.346 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Amicon Matrix Gel Red A gel filtration Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.274 2,5-didehydro-D-gluconate + NADPH
-
 Corynebacterium sp. 2-keto-L-gulonate + NADP+
-
 ?
1.1.1.274 2,5-didehydro-D-gluconate + NADPH
-
 Corynebacterium sp. SHS 0007 2-keto-L-gulonate + NADP+
-
 ?
1.1.1.274 5-keto-D-fructose + NADPH isoenzymes I and II Corynebacterium sp. L-sorbose + NADP+
-
 ?
1.1.1.274 5-keto-D-fructose + NADPH isoenzymes I and II Corynebacterium sp. SHS 0007 L-sorbose + NADP+
-
 ?
1.1.1.274 dihydroxyacetone + NADPH
-
 Corynebacterium sp. glycerol + NADP+
-
 ?
1.1.1.274 dihydroxyacetone + NADPH
-
 Corynebacterium sp. SHS 0007 glycerol + NADP+
-
 ?
1.1.1.346 2,5-didehydro-D-gluconate + NADPH + H+ 100% activity Corynebacterium sp. 2-dehydro-L-gulonate + NADP+
-
 ?
1.1.1.346 2,5-didehydro-D-gluconate + NADPH + H+ 100% activity Corynebacterium sp. SHS 0007 2-dehydro-L-gulonate + NADP+
-
 ?
1.1.1.346 5-dehydro-D-fructose + NADPH + H+ isoforms 2,5-diketo-D-gluconate reductase I and II show 150% and 13% activity, respectively, compared to 2,5-didehydro-D-gluconate Corynebacterium sp. L-sorbose + NADP+
-
 ?
1.1.1.346 additional information no activity with 2-dehydro-L-gulonate, 2-dehydro-D-gluconate, 5-dehydro-D-gluconate, D-fructose, and L-sorbose Corynebacterium sp. ?
-
 ?
1.1.1.346 additional information no activity with 2-dehydro-L-gulonate, 2-dehydro-D-gluconate, 5-dehydro-D-gluconate, D-fructose, and L-sorbose Corynebacterium sp. SHS 0007 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.274 monomer isoenzymes I and II Corynebacterium sp.
1.1.1.346 monomer 1 * 29000, isoform 2,5-diketo-D-gluconate reductase I, SDS-PAGE Corynebacterium sp.
1.1.1.346 monomer 1 * 34000, isoform 2,5-diketo-D-gluconate reductase II, SDS-PAGE Corynebacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.346 2,5-diketo-D-gluconate reductase I isoform Corynebacterium sp.
1.1.1.346 2,5-diketo-D-gluconate reductase II isoform Corynebacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.346 40
-
 isoform 2,5-diketo-D-gluconate reductase I Corynebacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.346
-
 38 temperature stability of isoform 2,5-diketo-D-gluconate reductase I is measured by storing the enzyme in 0.1 M Tris-HC1 buffer (pH 7.0) for 1 h. The activity of the enzyme decreases gradually with temperature from 0°C to 27°C, falls rapidly above 27°C, and is lost completely at about 38°C. The remaining activity at 27°C is about 77% of that at 0°C Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.274 6 7 isoenzyme I Corynebacterium sp.
1.1.1.274 6 7.5 isoenzyme II Corynebacterium sp.
1.1.1.346 6 7 isoform 2,5-diketo-D-gluconate reductase I Corynebacterium sp.
1.1.1.346 6 7.5 isoform 2,5-diketo-D-gluconate reductase II Corynebacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.346 5 7 when isoform I is incubated at different pH values for 3 h at 30°C, the remaining activity is about 29% of the original level at pH 5.0, about 65% at pH 6.0 and about 51% at pH 7.0 Corynebacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.274 NADP+
-
 Corynebacterium sp.
1.1.1.274 NADPH
-
 Corynebacterium sp.
1.1.1.346 NADPH
-
 Corynebacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.346 Corynebacterium sp. isoform 2,5-diketo-D-gluconate reductase II, isoelectric focusing
-
 4.1
1.1.1.346 Corynebacterium sp. isoform 2,5-diketo-D-gluconate reductase I, isoelectric focusing
-
 4.3