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Literature summary extracted from

  • Abken, H.J.; Tietze, M.; Brodersen, J.; Baumer, S.; Beifuss, U.; Deppenmeier, U.
    Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Go1 (1998), J. Bacteriol., 180, 2027-2032.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.3 0.035
-
 2-hydroxyphenazine at pH 7.0 and 25°C Methanosarcina mazei
1.5.98.3 0.25
-
 phenazine at pH 7.0 and 25°C Methanosarcina mazei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.98.3 membrane
-
 Methanosarcina mazei 16020
-
1.8.98.1 membrane bound to Methanosarcina mazei 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.98.3 reduced coenzyme F420 + methanophenazine Methanosarcina mazei
-
 oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.8.98.1 coenzyme B + coenzyme M + methanophenazine Methanosarcina mazei
-
 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine Methanosarcina mazei
-
 2-dihydropentaprenyloxyphenazine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.3 Methanosarcina mazei
-
-
-
1.8.98.1 Methanosarcina mazei
-
-
-
1.8.98.1 Methanosarcina mazei
-
 Gö1
-
1.12.98.3 Methanosarcina mazei
-
 strain Gö1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.3 1.8
-
 with phenazine as cosubstrate, at pH 7.0 and 25°C Methanosarcina mazei
1.5.98.3 4.6
-
 with phenazine-1-carboxylic acid as cosubstrate, at pH 7.0 and 25°C Methanosarcina mazei
1.5.98.3 8.4
-
 with 2-bromophenazine as cosubstrate, at pH 7.0 and 25°C Methanosarcina mazei
1.5.98.3 8.8
-
 with 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 25°C Methanosarcina mazei
1.5.98.3 10
-
 with methyl viologen as cosubstrate, at pH 7.0 and 25°C Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.3 reduced coenzyme F420 + 2-bromophenazine
-
 Methanosarcina mazei ?
-
 ?
1.5.98.3 reduced coenzyme F420 + 2-hydroxyphenazine
-
 Methanosarcina mazei ?
-
 ?
1.5.98.3 reduced coenzyme F420 + methanophenazine
-
 Methanosarcina mazei oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.5.98.3 reduced coenzyme F420 + methyl viologen
-
 Methanosarcina mazei ?
-
 ?
1.5.98.3 reduced coenzyme F420 + phenazine
-
 Methanosarcina mazei ?
-
 ?
1.5.98.3 reduced coenzyme F420 + phenazine-1-carboxylic acid
-
 Methanosarcina mazei ?
-
 ?
1.8.98.1 coenzyme B + coenzyme M + methanophenazine
-
 Methanosarcina mazei N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine
-
 ?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced 2-bromophenazine
-
 Methanosarcina mazei coenzyme B + coenzyme M + 2-bromophenazine
-
 ?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced 2-hydroxyphenazine
-
 Methanosarcina mazei coenzyme B + coenzyme M + 2-hydroxyphenazine
-
 ?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen
-
 Methanosarcina mazei coenzyme B + coenzyme M + methyl viologen
-
 ?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced phenazine-1-carboxylic acid
-
 Methanosarcina mazei coenzyme B + coenzyme M + phenazine-1-carboxylic acid
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine
-
 Methanosarcina mazei 2-dihydropentaprenyloxyphenazine
-
 ?
1.12.98.3 H2 + 2-hydroxyphenazine
-
 Methanosarcina mazei dihydro-2-hydroxyphenazine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.98.3 F420H2 dehydrogenase
-
 Methanosarcina mazei
1.5.98.3 fpoBCDIF
-
 Methanosarcina mazei
1.12.98.3 F420 non-reducing hydrogenase
-
 Methanosarcina mazei