Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Walker, K.; Croteau, R.
    Taxol biosynthetic genes (2001), Phytochemistry, 58, 1-7.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.176 medicine enzyme may be useful in the biological production of the potent antimitotic drug taxol which shows activity against a range of cancers Taxus cuspidata
1.14.14.176 medicine enzyme may be useful in the biological production of the potent antimitotic drug taxol which shows activity against a range of cancers Taxus brevifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.176 taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 Taxus cuspidata
-
 taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.176 taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 Taxus brevifolia
-
 taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.3.1.162 additional information Taxus cuspidata catalyzes the first acylation step of taxol biosynthesis ?
-
 ?
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III Taxus cuspidata
-
 CoA + baccatin III
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.176 Taxus brevifolia
-
-
-
1.14.14.176 Taxus cuspidata
-
-
-
2.3.1.162 Taxus cuspidata Q9M6F0
-
-
2.3.1.167 Taxus cuspidata Q9M6E2
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.162 acetyl-CoA + taxa-4(20),11-dien-5alpha-ol = CoA + taxa-4(20),11-dien-5alpha-yl acetate summary of taxol biosynthesis Taxus cuspidata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.167 leaf
-
 Taxus cuspidata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.176 taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2
-
 Taxus cuspidata taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.176 taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2
-
 Taxus brevifolia taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.3.1.162 acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
-
 Taxus cuspidata CoA + taxa-4(20),11-dien-5alpha-yl acetate
-
 ?
2.3.1.162 additional information catalyzes the first acylation step of taxol biosynthesis Taxus cuspidata ?
-
 ?
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus cuspidata CoA + baccatin III
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.167 7.4
-
-
 Taxus cuspidata