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Literature summary extracted from

  • Reddy, C.C.; Vaidyanathan, C.S.
    Purification, properties and induction of a specific benzoate-4-hydroxylase from Aspergillus niger (UBC 814) (1975), Biochim. Biophys. Acta, 384, 46-57.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.92 ascorbic acid 30% of activity with NADPH as electron donor Aspergillus niger

General Stability

EC Number General Stability Organism
1.14.14.92 benzoate stabilizes Aspergillus niger
1.14.14.92 EDTA stabilizes Aspergillus niger
1.14.14.92 glutathione stabilizes Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.92 2,2'-dipyridyl 0.05 mM; 50% inhibition Aspergillus niger
1.14.14.92 8-hydroxyquinoline 5 mM, complete inhibition Aspergillus niger
1.14.14.92 Cu2+ 0.001-0.01 mM, complete inhibition Aspergillus niger
1.14.14.92 diethyldithiocarbamate 5 mM, 95% inhibition Aspergillus niger
1.14.14.92 Hg2+ 0.001-0.01 mM, complete inhibition Aspergillus niger
1.14.14.92 Mg2+ 0.001-0.01 mM, complete inhibition Aspergillus niger
1.14.14.92 Mn2+ 0.001-0.01 mM, complete inhibition Aspergillus niger
1.14.14.92 Mo2+ 0.001-0.01 mM, complete inhibition Aspergillus niger
1.14.14.92 N-ethylmaleimide
-
Aspergillus niger
1.14.14.92 o-phenanthroline 0.05 mM, 70% inhibition Aspergillus niger
1.14.14.92 oxalate 2 mM, 20% inhibition Aspergillus niger
1.14.14.92 p-chloromercuribenzoate
-
Aspergillus niger
1.14.14.92 Zn2+ 0.001-0.01 mM, complete inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.92 0.13
-
benzoate
-
Aspergillus niger
1.14.14.92 0.16
-
NADPH
-
Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.92 Fe2+ required for activity Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.92 Aspergillus niger
-
-
-
1.14.14.92 Aspergillus niger UBC 814
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.92 protamine sulfate, tricalcium phosphate gel, DEAE-cellulose, alumina C-gamma gel Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.92 mycelium
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.92 0.0888
-
-
Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.14.14.92 4°C, 24 h, 40% loss of activity Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.92 benzoate + NADPH + H+ + O2
-
Aspergillus niger 4-hydroxybenzoate + NADP+ + H2O
-
r
1.14.14.92 benzoate + NADPH + H+ + O2
-
Aspergillus niger UBC 814 4-hydroxybenzoate + NADP+ + H2O
-
r
1.14.14.92 benzoate + [reduced NADPH-hemoprotein reductase] + O2 specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor Aspergillus niger 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.92 benzoate + [reduced NADPH-hemoprotein reductase] + O2 specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor Aspergillus niger UBC 814 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.92 30
-
-
Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.14.92 25 38 rapid decrease of activity above 38°C and below 25°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.92 6.2
-
-
Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.92 4.5 8 approx. 35% of maximal activity at pH 4.5, approx. 15% of maximal activity at pH 8.0 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.14.92 5.5 7.5
-
Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.92 tetrahydropteridine required as prosthetic group, maximal activity with 6,7-dimethyl 5,6,7,8-tetrahydropteridine, 60% activity with biopterine and tetrahydrofolic acid Aspergillus niger