EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.92 | ascorbic acid | 30% of activity with NADPH as electron donor | Aspergillus niger |
EC Number | General Stability | Organism |
---|---|---|
1.14.14.92 | benzoate stabilizes | Aspergillus niger |
1.14.14.92 | EDTA stabilizes | Aspergillus niger |
1.14.14.92 | glutathione stabilizes | Aspergillus niger |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.92 | 2,2'-dipyridyl | 0.05 mM; 50% inhibition | Aspergillus niger | |
1.14.14.92 | 8-hydroxyquinoline | 5 mM, complete inhibition | Aspergillus niger | |
1.14.14.92 | Cu2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
1.14.14.92 | diethyldithiocarbamate | 5 mM, 95% inhibition | Aspergillus niger | |
1.14.14.92 | Hg2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
1.14.14.92 | Mg2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
1.14.14.92 | Mn2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
1.14.14.92 | Mo2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger | |
1.14.14.92 | N-ethylmaleimide | - |
Aspergillus niger | |
1.14.14.92 | o-phenanthroline | 0.05 mM, 70% inhibition | Aspergillus niger | |
1.14.14.92 | oxalate | 2 mM, 20% inhibition | Aspergillus niger | |
1.14.14.92 | p-chloromercuribenzoate | - |
Aspergillus niger | |
1.14.14.92 | Zn2+ | 0.001-0.01 mM, complete inhibition | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.92 | 0.13 | - |
benzoate | - |
Aspergillus niger | |
1.14.14.92 | 0.16 | - |
NADPH | - |
Aspergillus niger |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.92 | Fe2+ | required for activity | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.92 | Aspergillus niger | - |
- |
- |
1.14.14.92 | Aspergillus niger UBC 814 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.92 | protamine sulfate, tricalcium phosphate gel, DEAE-cellulose, alumina C-gamma gel | Aspergillus niger |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.92 | mycelium | - |
Aspergillus niger | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.92 | 0.0888 | - |
- |
Aspergillus niger |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.14.92 | 4°C, 24 h, 40% loss of activity | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.92 | benzoate + NADPH + H+ + O2 | - |
Aspergillus niger | 4-hydroxybenzoate + NADP+ + H2O | - |
r | |
1.14.14.92 | benzoate + NADPH + H+ + O2 | - |
Aspergillus niger UBC 814 | 4-hydroxybenzoate + NADP+ + H2O | - |
r | |
1.14.14.92 | benzoate + [reduced NADPH-hemoprotein reductase] + O2 | specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor | Aspergillus niger | 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.92 | benzoate + [reduced NADPH-hemoprotein reductase] + O2 | specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor | Aspergillus niger UBC 814 | 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.92 | 30 | - |
- |
Aspergillus niger |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.92 | 25 | 38 | rapid decrease of activity above 38°C and below 25°C | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.92 | 6.2 | - |
- |
Aspergillus niger |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.92 | 4.5 | 8 | approx. 35% of maximal activity at pH 4.5, approx. 15% of maximal activity at pH 8.0 | Aspergillus niger |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.92 | 5.5 | 7.5 | - |
Aspergillus niger |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.92 | tetrahydropteridine | required as prosthetic group, maximal activity with 6,7-dimethyl 5,6,7,8-tetrahydropteridine, 60% activity with biopterine and tetrahydrofolic acid | Aspergillus niger |