Any feedback?
Literature summary extracted from
- Enzymic biosynthesis of vomilenine, a key intermediate of the ajmaline pathway, catalyzed by a novel cytochrome P450-dependent enzyme from plant cell cultures of Rauwolfia serpentina (1995), Z. Naturforsch. C, 50, 45-53.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.104 | CO | - |
Rauvolfia serpentina |  |
| 1.14.14.104 | cytochrome c | - |
Rauvolfia serpentina | |
| 1.14.14.104 | ketoconazole | - |
Rauvolfia serpentina | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.104 | 26 | - |
vinorine | - |
Rauvolfia serpentina | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.104 | vinorine + [reduced NADPH-hemoprotein reductase] + O2 | Rauvolfia serpentina | enzyme is involved in the biosynthesis of the alkaloid ajmaline | vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.104 | Rauvolfia serpentina | - |
- |
- |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.104 | -28°C, 20% sucrose, no loss of activity | Rauvolfia serpentina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.104 | vinorine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rauvolfia serpentina | vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.104 | vinorine + [reduced NADPH-hemoprotein reductase] + O2 | enzyme is involved in the biosynthesis of the alkaloid ajmaline | Rauvolfia serpentina | vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.104 | 40 | - |
- |
Rauvolfia serpentina |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.104 | 8.3 | - |
- |
Rauvolfia serpentina |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.104 | cytochrome P-450 | - |
Rauvolfia serpentina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
