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Literature summary extracted from

  • Vazquez-Flota, F.; De Carolis, E.; Alarco, A.M.; De Luca, V.
    Molecular cloning and characterization of desacetoxyvindoline-4-hydroxylase, a 2-oxoglutarate dependent-dioxygenase involved in the biosynthesis of vindoline in Catharanthus roseus (L.) G. Don (1997), Plant Mol. Biol., 34, 935-948.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.20 cloning of cDNA, expression in Escherichia coli, possibly dimorphic allele of a single-copy gene Catharanthus roseus
1.14.11.20 cloning of cDNA, possibly dimorphic allele of a single-copy gene Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.20 45647
-
 * 45647, calculated Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.20 Catharanthus roseus O04847
-
-
1.14.11.20 Catharanthus roseus O04847 (L.) G. Don
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.20 fruit low expression Catharanthus roseus
-
1.14.11.20 fruit 5% of activity in leaf Catharanthus roseus
-
1.14.11.20 leaf highest activity in leaf Catharanthus roseus
-
1.14.11.20 leaf predominantly in young leaf Catharanthus roseus
-
1.14.11.20 seed etiolated seed, contains considerable levels of transcript but almost undetectable enzymic activity. Exposure of seedlings to light results in a rapid increase of enzyme activity without a significant further increase in d4h transcripts over those detected in dark-grown seedlings Catharanthus roseus
-
1.14.11.20 stem low expression Catharanthus roseus
-
1.14.11.20 stem 8% of activity in leaf Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.20 deacetoxyvindoline + 2-oxoglutarate + O2
-
 Catharanthus roseus deacetylvindoline + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.20 ? * 45647, calculated Catharanthus roseus

Synonyms

EC Number Synonyms Comment Organism
1.14.11.20 D4H
-
 Catharanthus roseus