EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | dithiothreitol | - |
Bos taurus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.39 | expression of endothelial isoform in human embryonic kidney cells | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | EDTA | inhibits at concentrations above 0.01 mM | Bos taurus | |
1.14.13.39 | Ngamma-nitro-L-arginine methyl ester | complete inhibition | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.39 | 0.0021 | - |
L-arginine | - |
Bos taurus | |
1.14.13.39 | 0.019 | - |
Ngamma-hydroxy-L-arginine | - |
Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | Ca2+ | required | Bos taurus | |
1.14.13.39 | Iron | heme-iron | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.39 | Bos taurus | - |
endothelial isoform | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.39 | recombinant endothelial isoform from human embryonic kidney cells | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.39 | endothelium | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | 0.143 | - |
purified recombinant enzyme | Bos taurus |
1.14.13.39 | 0.41 | - |
substrate Ngamma-hydroxy-L-arginine, purified enzyme | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
1.14.13.39 | ferricyanide + NADPH + O2 | - |
Bos taurus | ferrocyanide + NADP+ + ? | - |
? | |
1.14.13.39 | Ngamma-hydroxy-L-arginine + NADPH + O2 | - |
Bos taurus | citrulline + NADP+ + NO | - |
? | |
1.14.13.39 | oxidized cytochrome c + NADPH + O2 | - |
Bos taurus | reduced cytochrome c + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.39 | dimer | 2 * 130000-150000, SDS-PAGE | Bos taurus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.39 | e-NOS | endothelial isoform III | Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | 25 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | 7.4 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Bos taurus | |
1.14.13.39 | Calmodulin | enzyme-bound is required, supplemented stimulates | Bos taurus | |
1.14.13.39 | FAD | - |
Bos taurus | |
1.14.13.39 | FMN | - |
Bos taurus |