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Literature summary extracted from

  • Richards, M.K.; Clague, M.J.; Marletta, M.A.
    Characterization of C415 mutants of neuronal nitric oxide synthase (1996), Biochemistry, 35, 7772-7780.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 dithiothreitol
-
 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 expression of neuronal isoform wild type and mutants C415H, C415A in Spodoptera frugiperda cells via baculovirus infection Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.39 C415A contains no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c Mus musculus
1.14.13.39 C415H contains nearly no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+
-
 Mus musculus
1.14.13.39 Iron heme ligand is bound via C415 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 wild-type and mutants from insect cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 macrophage
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 additional information
-
 wild-type and mutants Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 oxidized cytochrome c + NADPH + O2 wild-type and mutants Mus musculus reduced cytochrome c + NADP+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin only wild-type Mus musculus
1.14.13.39 Calmodulin Ca2+/calmodulin stimulates cytochrome c reductase activity Mus musculus
1.14.13.39 Calmodulin 15fold stimulation of cytochrome c reduction of wild-type and mutants C415A and C415H Mus musculus
1.14.13.39 FAD wild-type and mutant C415H contain1 mol per mol of subunit Mus musculus
1.14.13.39 FMN wild-type and mutant C415H contain 0.8 and 0.9 mol per mol of subunit, respectively Mus musculus
1.14.13.39 NADPH
-
 Mus musculus