BRENDA - Enzyme Database

Agmatine enhances the NADPH oxidase activity of neuronal NO synthase and leads to oxidative inactivation of the enzyme

Demady, D.R.; Jianmongkol, S.; Vuletich, J.L.; Bender, A.T.; Osawa, Y.; Mol. Pharmacol. 59, 24-29 (2001)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.14.13.39
pharmacology
NO synthase can be used to gain insight into the biological role of endogenous agmatine
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.39
neuronal enzyme expressed in Spodoptera frugiperda cells via baculovirus infection
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.39
agmatine
at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin; causes an increase in NADPH oxidase activity of the enzyme
Homo sapiens
1.14.13.39
H2O2
alters heme group, decrease in activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.13.39
cytosol
-
Homo sapiens
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Iron
0.88 mol per mol of recombinant enzyme monomer
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.39
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.39
wild-type recombinant from insect cells
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
neuron
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.39
additional information
-
assay method
Homo sapiens
1.14.13.39
1
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440233
Homo sapiens
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
440233
Homo sapiens
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.13.39
0.66
-
agmatine
at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
1.14.13.39
pharmacology
NO synthase can be used to gain insight into the biological role of endogenous agmatine
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.39
neuronal enzyme expressed in Spodoptera frugiperda cells via baculovirus infection
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.39
agmatine
at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin; causes an increase in NADPH oxidase activity of the enzyme
Homo sapiens
1.14.13.39
H2O2
alters heme group, decrease in activity
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.13.39
0.66
-
agmatine
at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.13.39
cytosol
-
Homo sapiens
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Iron
0.88 mol per mol of recombinant enzyme monomer
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.39
wild-type recombinant from insect cells
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
neuron
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.39
additional information
-
assay method
Homo sapiens
1.14.13.39
1
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440233
Homo sapiens
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
440233
Homo sapiens
?