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Literature summary extracted from

  • Campos, K.L.; Giovanelli, J.; Kaufman, S.
    Characteristics of the nitric oxide synthase-catalyzed conversion of arginine to N-hydroxyarginine, the first oxygenation step in the enzymic synthesis of nitric oxide (1995), J. Biol. Chem., 270, 1721-1728.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 rat brain enzyme expressed in human 293 kidney cells transfected with a vector encoding rat brain enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 cyanide pretreatment Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Iron
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 cerebellum wild-type and recombinant brain enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 brain
-
 Rattus norvegicus
-
1.14.13.39 cerebellum
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ the overall reaction proceeds via 2 partial reactions: reaction 1 converts L-arginine into L-Ngamma-hydroxyarginine, reaction 2 converts L-Ngamma-hydroxyarginine into citrulline and nitric oxide Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ir
1.14.13.39 Ngamma-hydroxy-L-arginine + NADPH + O2 substrate is intermediate between reaction 1 and 2 to form citrulline and NO from L-arginine Rattus norvegicus citrulline + NADP+ + NO
-
 ir

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin required Rattus norvegicus
1.14.13.39 NADPH
-
 Rattus norvegicus