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Literature summary extracted from

  • Iwasaki, T.; Hori, H.; Hayashi, Y.; Nishino, T.; Tamura, K.; Oue, S.; Iizuka, T.; Ogura, T.; Esumi, H.
    Characterization of mouse nNOS2, a natural variant of neuronal nitric-oxide synthase produced in the central nervous system by selective alternative splicing (1999), J. Biol. Chem., 274, 17559-17566.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 neuronal wild-type and natural mutant, specifically expressed in the central nervous system, expression in Escherichia coli and in insect cells of Spodoptera frugiperda via baculovirus infection Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.39 x-ray crystal structure of the heme-binding domains of neuronal wild-type and mutant with deletion in the heme-binding domain Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.39 additional information naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Iron naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin, the heme coordination geometry is highly abnormal Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 wild-type and natural mutant, recombinant from Escherichia coli and insect cells Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O alignment of heme-binding domain amino acid sequences of NOS Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 neuron central nervous system, natural variant with 105-amino acid deletion in the heme-binding domain Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 NADPH
-
 Mus musculus