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Literature summary extracted from
- Purification of a Ca2+/calmodulin-dependent nitric oxide synthase from porcine cerebellum. Cofactor-role of tetrahydrobiopterin (1990), FEBS Lett., 277, 215-219.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.39 | glycerol stabilizes | Sus scrofa |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | Ca2+ | activation only in the presence of calmodulin | Sus scrofa |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 160000 | - |
x * 160000, SDS-PAGE | Sus scrofa |
| 1.14.13.39 | 200000 | - |
above, gel filtration | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.39 | ammonium sulfate precipitation (30% saturation), 2',5'-ADP-affinity chromatography | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | brain | - |
Sus scrofa | - |
| 1.14.13.39 | cerebellum | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 0.73 | - |
purified enzyme | Sus scrofa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.39 | -70°C, with 20% v/v glycerol at least 4 weeks stable | Sus scrofa |
| 1.14.13.39 | 4°C, unstable | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Sus scrofa | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.39 | ? | x * 160000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 37 | - |
assay at | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Sus scrofa | |
| 1.14.13.39 | NADPH | - |
Sus scrofa | |
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Release 2023.1 (January 2023)
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