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Literature summary extracted from

  • Knowles, R.G.; Palacios, M.; Palmer, R.M.J.; Moncada, S.
    Kinetic characteristics of nitric oxide synthase from rat brain (1990), Biochem. J., 269, 207-210.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 Ngamma-iminoethyl-L-ornithine competitive inhibitor Rattus norvegicus
1.14.13.39 Ngamma-monomethyl-L-arginine not D-isomer, strong, competitive Rattus norvegicus
1.14.13.39 Ngamma-nitro-L-arginine competitive inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.008 0.0084 dichlorophenolindophenol with L-arginine Rattus norvegicus
1.14.13.39 0.0084
-
 L-arginine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.39 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 additional information Rattus norvegicus NO represents the endogenous activator of soluble guanylyl cyclase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 brain
-
 Rattus norvegicus
-
1.14.13.39 forebrain synaptosomal fraction Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 0.000043
-
 crude extract Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound ?
1.14.13.39 2,6-dichlorophenolindophenol + NADPH + O2
-
 Rattus norvegicus ? + NO + NADP+
-
 ?
1.14.13.39 additional information NO represents the endogenous activator of soluble guanylyl cyclase Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7.2
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 NADPH dependent on Rattus norvegicus
1.14.13.39 NADPH crude preparation requires only NADPH as cofactor Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.39 0.0004
-
 Ngamma-nitro-L-arginine
-
 Rattus norvegicus
1.14.13.39 0.0007
-
 Ngamma-monomethyl-L-arginine
-
 Rattus norvegicus
1.14.13.39 0.0012
-
 Ngamma-iminoethyl-L-ornithine
-
 Rattus norvegicus