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Literature summary extracted from

  • Yui, Y.; Hattori, R.; Kosuga, K.; Eizawa, H.;Hiki, K.; Ohkawa, S.; Ohnishi, K.; Terao, S.; Kawai, C.
    Calmodulin-independent nitric oxide synthase from rat polymorphonuclear neutrophils (1991), J. Biol. Chem., 266, 3369-3371.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.39 unstable during purification Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 additional information the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide; the macrophage enzyme is not inhibited by calmodulin antagonists (N-6-aminohexyl)-1-naphthalene sulfonamide Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.022
-
 L-arginine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.39 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ required, calmodulin independent Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.39 150000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 2',5'-ADP-affinity and anion exchange chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 macrophage
-
 Rattus norvegicus
-
1.14.13.39 neutrophil polymorphonuclear Rattus norvegicus
-
1.14.13.39 neutrophil peritoneal polymorphonuclear Rattus norvegicus
-
1.14.13.39 peritoneum
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 0.12
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.13.39 4°C, crude, stable for at least 24 h Rattus norvegicus
1.14.13.39 4°C, purified, t1/2: 3 h at pH 7.4 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O guanidino-nitrogen of L-arginine is oxidized to form NO and citrulline ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 monomer 1 * 150000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 additional information
-
 pI: 5.6 Rattus norvegicus
1.14.13.39 7 8
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.39 7.4
-
 purified, t1/2: 3 h, 4°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin required Rattus norvegicus
1.14.13.39 Calmodulin rat neutrophil enzyme is calmodulin independent Rattus norvegicus
1.14.13.39 FAD required Rattus norvegicus
1.14.13.39 NADPH
-
 Rattus norvegicus