Literature summary extracted from
Yui, Y.; Hattori, R.; Kosuga, K.; Eizawa, H.;Hiki, K.; Kawai, C.
Purification of nitric oxide synthase from rat macrophages (1991), J. Biol. Chem., 266, 12544-12547.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.14.13.39 |
dithiothreitol |
requirement |
Rattus norvegicus |
|
General Stability
EC Number |
General Stability |
Organism |
---|
1.14.13.39 |
dithiothreitol stabilizes |
Rattus norvegicus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.13.39 |
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid |
i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme |
Rattus norvegicus |
|
1.14.13.39 |
additional information |
the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide; the macrophage enzyme is not inhibited by calmodulin antagonists (N-6-aminohexyl)-1-naphthalene sulfonamide |
Rattus norvegicus |
|
1.14.13.39 |
Trifluoperazine |
no inhibitor of macrophage enzyme |
Rattus norvegicus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.14.13.39 |
0.0323 |
0.035 |
L-arginine |
- |
Rattus norvegicus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.13.39 |
150000 |
- |
2 * 150000, SDS-PAGE |
Rattus norvegicus |
1.14.13.39 |
300000 |
- |
gel filtration |
Rattus norvegicus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.13.39 |
Rattus norvegicus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.13.39 |
- |
Rattus norvegicus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.14.13.39 |
macrophage |
- |
Rattus norvegicus |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.13.39 |
0.94 |
0.96 |
purified enzyme |
Rattus norvegicus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.14.13.39 |
4°C, crude, stable for at least 24 h |
Rattus norvegicus |
1.14.13.39 |
4°C, purified, t1/2: 6 h at pH 7.4 |
Rattus norvegicus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.13.39 |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ |
- |
Rattus norvegicus |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.13.39 |
dimer |
2 * 150000, SDS-PAGE |
Rattus norvegicus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.13.39 |
37 |
- |
assay at |
Rattus norvegicus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.13.39 |
7 |
8 |
- |
Rattus norvegicus |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
1.14.13.39 |
7.4 |
- |
purified, t1/2: 6 h, 4°C |
Rattus norvegicus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.13.39 |
5,6,7,8-tetrahydro-L-biopterin |
required |
Rattus norvegicus |
|
1.14.13.39 |
FAD |
- |
Rattus norvegicus |
|
1.14.13.39 |
NADPH |
dependent on |
Rattus norvegicus |
|