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Literature summary extracted from

  • Yui, Y.; Hattori, R.; Kosuga, K.; Eizawa, H.;Hiki, K.; Kawai, C.
    Purification of nitric oxide synthase from rat macrophages (1991), J. Biol. Chem., 266, 12544-12547.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 dithiothreitol requirement Rattus norvegicus

General Stability

EC Number General Stability Organism
1.14.13.39 dithiothreitol stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme Rattus norvegicus
1.14.13.39 additional information the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide; the macrophage enzyme is not inhibited by calmodulin antagonists (N-6-aminohexyl)-1-naphthalene sulfonamide Rattus norvegicus
1.14.13.39 Trifluoperazine no inhibitor of macrophage enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.0323 0.035 L-arginine
-
Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.39 150000
-
2 * 150000, SDS-PAGE Rattus norvegicus
1.14.13.39 300000
-
gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 macrophage
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 0.94 0.96 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.13.39 4°C, crude, stable for at least 24 h Rattus norvegicus
1.14.13.39 4°C, purified, t1/2: 6 h at pH 7.4 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
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Subunits

EC Number Subunits Comment Organism
1.14.13.39 dimer 2 * 150000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 7 8
-
Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.39 7.4
-
purified, t1/2: 6 h, 4°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin required Rattus norvegicus
1.14.13.39 FAD
-
Rattus norvegicus
1.14.13.39 NADPH dependent on Rattus norvegicus