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Literature summary extracted from
- Purification and characterization of a human NO synthase (1991), Biochem. Biophys. Res. Commun., 181, 1372-1377.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | Calmidazolium | - |
Homo sapiens |  |
| 1.14.13.39 | Ngamma-monomethyl-L-arginine | - |
Homo sapiens | |
| 1.14.13.39 | Ngamma-nitro-L-arginine | - |
Homo sapiens | |
| 1.14.13.39 | Trifluoperazine | inhibition in the presence of Ca2+, reversible by calmodulin | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.39 | cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | Ca2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 160000 | - |
2 * 160000, SDS-PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.39 | affinity and size exclusion chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | brain | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 0.074 | - |
purified enzyme | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.39 | dimer | 2 * 160000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 37 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Homo sapiens | |
| 1.14.13.39 | Calmodulin | - |
Homo sapiens | |
| 1.14.13.39 | NADPH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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