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Literature summary extracted from

  • Hecker, M.; Walsh, D.T.; Vane, J.R.
    On the substrate specificity of nitric oxide synthase (1991), FEBS Lett., 294, 221-224.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme Bos taurus
1.14.13.39 ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme Mus musculus
1.14.13.39 Ngamma-monomethyl-L-arginine
-
 Bos taurus
1.14.13.39 Ngamma-monomethyl-L-arginine
-
 Mus musculus
1.14.13.39 Ngamma-nitro-L-arginine
-
 Bos taurus
1.14.13.39 Ngamma-nitro-L-arginine
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.39 cytosol
-
 Mus musculus 5829
-
1.14.13.39 cytosol
-
 Bos taurus 5829
-
1.14.13.39 membrane constitutive endothelial enzyme: predominantly membrane-bound, inducible macrophage enzyme: equally distributed between cytosol and membrane, small constitutive membrane-bound portion in murine macrophages Mus musculus 16020
-
1.14.13.39 membrane constitutive endothelial enzyme: predominantly membrane-bound, inducible macrophage enzyme: equally distributed between cytosol and membrane, small constitutive membrane-bound portion in murine macrophages Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ insoluble enzyme Mus musculus
1.14.13.39 Ca2+ insoluble enzyme Bos taurus
1.14.13.39 Ca2+ constitutive endothelial and inducible membrane bound macrophage enzyme are strictly Ca2+-dependent Mus musculus
1.14.13.39 Ca2+ constitutive endothelial and inducible membrane bound macrophage enzyme are strictly Ca2+-dependent Bos taurus
1.14.13.39 Ca2+ macrophage enzyme Mus musculus
1.14.13.39 Ca2+ macrophage enzyme Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Bos taurus
-
 3 isoforms exist in bovine aortic endothelial cells differentiated by substrate specificity and Ca2+-dependence
-
1.14.13.39 Mus musculus
-
 3 isoforms exist in mouse macrophages differentiated by substrate specificity and Ca2+-dependence
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 endothelium aorta, cell culture Mus musculus
-
1.14.13.39 endothelium aorta, cell culture Bos taurus
-
1.14.13.39 macrophage
-
 Mus musculus
-
1.14.13.39 macrophage
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Bos taurus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 L-Ala-L-Arg + NADPH + O2 endothelial microsomes, macrophage Mus musculus ?
-
 ?
1.14.13.39 L-Ala-L-Arg + NADPH + O2 endothelial microsomes, macrophage Bos taurus ?
-
 ?
1.14.13.39 L-Arg-L-Arg + NADPH + O2 endothelial, microsomes, macrophage Mus musculus ?
-
 ?
1.14.13.39 L-Arg-L-Arg + NADPH + O2 endothelial, microsomes, macrophage Bos taurus ?
-
 ?
1.14.13.39 L-Arg-L-Arg-L-Arg + NADPH + O2 endothelial microsomes Mus musculus ?
-
 ?
1.14.13.39 L-Arg-L-Arg-L-Arg + NADPH + O2 endothelial microsomes Bos taurus ?
-
 ?
1.14.13.39 L-Arg-L-Phe + NADPH + O2
-
 Mus musculus ?
-
 ?
1.14.13.39 L-Arg-L-Phe + NADPH + O2
-
 Bos taurus ?
-
 ?
1.14.13.39 L-homoarginine + NADPH + O2 constitutive endothelial membrane-bound and inducible soluble macrophage enzyme Mus musculus ?
-
 ?
1.14.13.39 L-homoarginine + NADPH + O2 constitutive endothelial membrane-bound and inducible soluble macrophage enzyme Bos taurus ?
-
 ?
1.14.13.39 Ngamma-hydroxy-L-arginine + NADPH + O2 best substrate Mus musculus citrulline + NADP+ + NO
-
 ?
1.14.13.39 Ngamma-hydroxy-L-arginine + NADPH + O2 best substrate Bos taurus citrulline + NADP+ + NO
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Mus musculus
1.14.13.39 37
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 NADPH NADPH-dependent dioxygenase Mus musculus
1.14.13.39 NADPH NADPH-dependent dioxygenase Bos taurus