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Literature summary extracted from

  • Stuehr, D.J.; Cho, H.J.; Kwon, N.S.; Weise, M.F.; Nathan, C.F.
    Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMN-containing flavoprotein (1991), Proc. Natl. Acad. Sci. USA, 88, 7773-7777.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 interferon gamma activates Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 EDTA no inhibition Mus musculus
1.14.13.39 ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme Mus musculus
1.14.13.39 additional information the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.0003
-
 NADPH
-
 Mus musculus
1.14.13.39 0.002 0.0028 L-arginine
-
 Mus musculus
1.14.13.39 0.0028
-
 L-arginine
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+ slight activation Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.39 additional information
-
 dissociates irreversibly into subunits in the absence of L-arginine, FAD and tetrahydrobiopterin Mus musculus
1.14.13.39 130000
-
 2 * 130000, SDS-PAGE Mus musculus
1.14.13.39 250000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Mus musculus enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 gel filtration chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 macrophage Ca2+-independent form Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 1.06
-
 purified enzyme Mus musculus

Storage Stability

EC Number Storage Stability Organism
1.14.13.39 -80°C, diluted preparation stable overnight Mus musculus
1.14.13.39 4°C, 6 h stable Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 dimer 2 * 130000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.39 37
-
 t1/2: about 6 h Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin
-
 Mus musculus
1.14.13.39 Calmodulin murine macrophage enzyme is Ca2+/calmodulin independent Mus musculus
1.14.13.39 FAD 2.2 mol FAD per mol of enzyme dimer Mus musculus
1.14.13.39 FAD non-covalently bound FAD Mus musculus
1.14.13.39 FMN 1.1 mol FMN per mol enzyme dimer Mus musculus
1.14.13.39 NADPH
-
 Mus musculus