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Literature summary extracted from

  • Bredt, D.S.; Snyder, S.H.
    Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme (1990), Proc. Natl. Acad. Sci. USA, 87, 682-685.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.14.13.39 bovine serum albumin stabilizes Rattus norvegicus
1.14.13.39 glycerol stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamide
-
 Rattus norvegicus
1.14.13.39 N-(6-Aminohexyl)-1-naphthalene sulfonamide
-
 Rattus norvegicus
1.14.13.39 Trifluoperazine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.002 0.0028 L-arginine
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Ca2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.39 150000
-
 1 * 150000, SDS-PAGE Rattus norvegicus
1.14.13.39 200000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 2',5'-ADP-affinity and anion exchange chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 0.94 0.96
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.13.39 0°C, crude, t1/2: 2 days, purified t1/2: 2 h, with 1 mg/ml bovine serum albumin and 20% v/v glycerol as stabilizing agents, t1/2: 7 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 monomer 1 * 150000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 Calmodulin activation, potent stimulator of purified, not crude, enzyme preparation Rattus norvegicus
1.14.13.39 NADPH
-
 Rattus norvegicus