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Literature summary extracted from

  • Zumft, W.G.; Mortenson, L.E.
    The nitrogen-fixing complex of bacteria (1975), Biochim. Biophys. Acta, 416, 1-52.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.6.1 additional information overview Azotobacter vinelandii
1.18.6.1 additional information overview Clostridium pasteurianum
1.18.6.1 additional information overview Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.6.1 additional information
-
 additional information overview, different substrates Klebsiella pneumoniae
1.18.6.1 additional information
-
 additional information overview, different substrates Clostridium pasteurianum
1.18.6.1 additional information
-
 additional information overview, different substrates Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron
-
 Klebsiella pneumoniae
1.18.6.1 Iron
-
 Clostridium pasteurianum
1.18.6.1 Iron
-
 Azotobacter vinelandii
1.18.6.1 Iron
-
 Chromatium sp.
1.18.6.1 Iron
-
 Rhizobium sp.
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Klebsiella pneumoniae
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Clostridium pasteurianum
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Azotobacter vinelandii
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Chromatium sp.
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Rhizobium sp.
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Klebsiella pneumoniae
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Clostridium pasteurianum
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Azotobacter vinelandii
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Chromatium sp.
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Rhizobium sp.
1.18.6.1 additional information
-
 Klebsiella pneumoniae
1.18.6.1 additional information
-
 Azotobacter vinelandii
1.18.6.1 additional information
-
 Chromatium sp.
1.18.6.1 additional information
-
 Rhizobium sp.
1.18.6.1 additional information contains also an inactive MoFe protein species Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 additional information
-
 comparison of amino acid composition Klebsiella pneumoniae
1.18.6.1 additional information
-
 comparison of amino acid composition Clostridium pasteurianum
1.18.6.1 additional information
-
 comparison of amino acid composition Azotobacter vinelandii
1.18.6.1 35000 40000 Fe protein Azotobacter vinelandii
1.18.6.1 55000 60000 component II Fe protein Klebsiella pneumoniae
1.18.6.1 55000 60000 component II Fe protein Clostridium pasteurianum
1.18.6.1 55000 60000 component II Fe protein Azotobacter vinelandii
1.18.6.1 66800
-
 Fe protein Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Chromatium sp.
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhizobium sp.
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter vinelandii
-
-
-
1.18.6.1 Chromatium sp.
-
-
-
1.18.6.1 Clostridium pasteurianum
-
-
-
1.18.6.1 Klebsiella pneumoniae
-
-
-
1.18.6.1 Rhizobium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1
-
 Azotobacter vinelandii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Clostridium pasteurianum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Azotobacter vinelandii
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Chromatium sp.
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Rhizobium sp.
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Chromatium sp.
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhizobium sp.
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate schematic mechanism Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate schematic mechanism Clostridium pasteurianum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate schematic mechanism Azotobacter vinelandii
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate schematic mechanism Chromatium sp.
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate schematic mechanism Rhizobium sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.1 additional information
-
 overview Klebsiella pneumoniae
1.18.6.1 additional information
-
 overview Clostridium pasteurianum
1.18.6.1 additional information
-
 overview Azotobacter vinelandii
1.18.6.1 additional information
-
 overview Chromatium sp.
1.18.6.1 additional information
-
 overview Rhizobium sp.
1.18.6.1 additional information
-
 activity of Fe proteins and MoFe proteins Klebsiella pneumoniae
1.18.6.1 additional information
-
 activity of Fe proteins and MoFe proteins Clostridium pasteurianum
1.18.6.1 additional information
-
 activity of Fe proteins and MoFe proteins Azotobacter vinelandii
1.18.6.1 additional information
-
 activity of Fe proteins and MoFe proteins Chromatium sp.
1.18.6.1 additional information
-
 activity of Fe proteins and MoFe proteins Rhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Klebsiella pneumoniae 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Clostridium pasteurianum 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Chromatium sp. 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Rhizobium sp. 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Chromatium sp. 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhizobium sp. 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 dithionite + H+ + N2 + ATP in vitro substrate Klebsiella pneumoniae ?
-
 ?
1.18.6.1 dithionite + H+ + N2 + ATP in vitro substrate Clostridium pasteurianum ?
-
 ?
1.18.6.1 dithionite + H+ + N2 + ATP in vitro substrate Azotobacter vinelandii ?
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CH3NC + ATP
-
 Klebsiella pneumoniae oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CH3NC + ATP
-
 Clostridium pasteurianum oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CH3NC + ATP
-
 Azotobacter vinelandii oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CH3NC + ATP
-
 Chromatium sp. oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CH3NC + ATP
-
 Rhizobium sp. oxidized ferredoxin + CH4 + C2H4 + C3H6 + C3H8 + CH3NH2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CN- + ATP
-
 Klebsiella pneumoniae oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CN- + ATP
-
 Clostridium pasteurianum oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CN- + ATP
-
 Azotobacter vinelandii oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CN- + ATP
-
 Chromatium sp. oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + CN- + ATP
-
 Rhizobium sp. oxidized ferredoxin + CH4 + NH3 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + N3- + ATP
-
 Klebsiella pneumoniae oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + N3- + ATP
-
 Clostridium pasteurianum oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + N3- + ATP
-
 Azotobacter vinelandii oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + N3- + ATP
-
 Chromatium sp. oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
 ?
1.18.6.1 reduced ferredoxin + H+ + N3- + ATP
-
 Rhizobium sp. oxidized ferredoxin + NH3 + N2 + ADP + phosphate
-
 ?