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Literature summary extracted from

  • Hallenbeck, P.C.; Meyer, C.; Vignais, P.M.
    Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata: purification and molecular properties (1982), J. Bacteriol., 149, 708-717.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.18.6.1 additional information Fe protein contains an adenine-like molecule, a pentose moiety and a phosphate residue covalently attached to the molecule Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron
-
 Rhodobacter capsulatus
1.18.6.1 Molybdenum
-
 Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 additional information
-
-
 Rhodobacter capsulatus
1.18.6.1 33500
-
 MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus
1.18.6.1 55000
-
 MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus
1.18.6.1 59000
-
 MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus
1.18.6.1 63000
-
 Fe protein Rhodobacter capsulatus
1.18.6.1 230000
-
 MoFe protein Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhodobacter capsulatus
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1
-
 Rhodobacter capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhodobacter capsulatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.1 0.6 0.8 purified Fe protein Rhodobacter capsulatus
1.18.6.1 1.5 1.7 purified MoFe protein Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhodobacter capsulatus 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.6.1 More
-
 Rhodobacter capsulatus
1.18.6.1 tetramer MoFe protein 1 * 55000 + 1 * 59000 + Fe protein 2 * 33500, SDS-PAGE Rhodobacter capsulatus