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The nitrogen-fixing system of Corynebacterium autotrophicum. Purification and properties of the nitrogenase components and two ferredoxins

Berndt, H.; Lowe, D.J.; Yates, M.G.; Eur. J. Biochem. 86, 133-142 (1978)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Xanthobacter autotrophicus
1.18.6.1
Molybdenum
-
Xanthobacter autotrophicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
additional information
-
-
Xanthobacter autotrophicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Xanthobacter autotrophicus
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Xanthobacter autotrophicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.6.1
both components
Xanthobacter autotrophicus
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Xanthobacter autotrophicus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
1.07
-
purified MoFe protein
Xanthobacter autotrophicus
1.18.6.1
1.26
-
purified Fe protein
Xanthobacter autotrophicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440168
Xanthobacter autotrophicus
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
440168
Xanthobacter autotrophicus
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440168
Xanthobacter autotrophicus
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440168
Xanthobacter autotrophicus
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440168
Xanthobacter autotrophicus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.18.6.1
More
-
Xanthobacter autotrophicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.18.6.1
7.1
7.3
with substrates: N2, C2H2
Xanthobacter autotrophicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.18.6.1
6.5
8.3
pH 6.5: no activity below, pH 8.3: about 70% of activity maximum
Xanthobacter autotrophicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Xanthobacter autotrophicus
1.18.6.1
Molybdenum
-
Xanthobacter autotrophicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
additional information
-
-
Xanthobacter autotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Xanthobacter autotrophicus
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.6.1
both components
Xanthobacter autotrophicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
1.07
-
purified MoFe protein
Xanthobacter autotrophicus
1.18.6.1
1.26
-
purified Fe protein
Xanthobacter autotrophicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440168
Xanthobacter autotrophicus
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
440168
Xanthobacter autotrophicus
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440168
Xanthobacter autotrophicus
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440168
Xanthobacter autotrophicus
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440168
Xanthobacter autotrophicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.18.6.1
More
-
Xanthobacter autotrophicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.18.6.1
7.1
7.3
with substrates: N2, C2H2
Xanthobacter autotrophicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.18.6.1
6.5
8.3
pH 6.5: no activity below, pH 8.3: about 70% of activity maximum
Xanthobacter autotrophicus