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Literature summary extracted from

  • Whitting, M.J.; Dilworth, M.J.
    Legume root nodule nitrogenase. Purification, properties, and studies on its genetic control (1974), Biochim. Biophys. Acta, 371, 337-351.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron 4 atoms of iron per molecule of Fe protein Bradyrhizobium lupini
1.18.6.1 Molybdenum
-
Bradyrhizobium lupini

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 additional information
-
-
Bradyrhizobium lupini
1.18.6.1 65000
-
Fe protein, gel filtration Bradyrhizobium lupini
1.18.6.1 194000
-
MoFe protein, gel filtration Bradyrhizobium lupini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Bradyrhizobium lupini
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Bradyrhizobium lupini
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.18.6.1 extreme O2 lability, t1/2: 1 min, Fe protein Bradyrhizobium lupini

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1 both components Bradyrhizobium lupini

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Bradyrhizobium lupini

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.6.1 root nodule bacteroid Bradyrhizobium lupini
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.1 0.43
-
purified Fe protein Bradyrhizobium lupini
1.18.6.1 0.7
-
purified MoFe protein Bradyrhizobium lupini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Bradyrhizobium lupini 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?