BRENDA - Enzyme Database show

Large-scale purification of high activity Azotobacter vinelandii nitrogenase

Burgess, B.K.; Jacobs, D.B.; Stiefel, E.I.; Biochim. Biophys. Acta 614, 196-209 (1980)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Azotobacter vinelandii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.6.1
large scale; strict anaerobic conditions
Azotobacter vinelandii
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Azotobacter vinelandii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.18.6.1
1.01
-
purified MoFe protein
Azotobacter vinelandii
1.18.6.1
2.01
-
purified Fe protein
Azotobacter vinelandii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440163
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440163
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440163
Azotobacter vinelandii
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.18.6.1
30
-
assay at
Azotobacter vinelandii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.18.6.1
7.4
-
assay at
Azotobacter vinelandii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.6.1
large scale; strict anaerobic conditions
Azotobacter vinelandii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.18.6.1
1.01
-
purified MoFe protein
Azotobacter vinelandii
1.18.6.1
2.01
-
purified Fe protein
Azotobacter vinelandii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440163
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440163
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440163
Azotobacter vinelandii
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.18.6.1
30
-
assay at
Azotobacter vinelandii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.18.6.1
7.4
-
assay at
Azotobacter vinelandii