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Literature summary extracted from

  • Kush, A.; Elmerich, C.; Aubert, J.P.
    Nitrogenase of Sesbania Rhizobium strain ORS571: purification, properties, and 'switch-off' by ammonia (1985), J. Gen. Microbiol., 131, 1765-1777.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron 22.5 atoms of iron per molecule of protein Rhizobium sp.
1.18.6.1 Iron 3.1 Rhizobium sp.
1.18.6.1 Molybdenum 1.2-1.3 gatom per mol of MoFe protein Rhizobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 36000
-
 2 * 36000, SDS-PAGE Rhizobium sp.
1.18.6.1 74000
-
 Fe protein, gel filtration Rhizobium sp.
1.18.6.1 219000
-
 MoFe protein, gel filtration Rhizobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Rhizobium sp.
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Rhizobium sp.
-
 ORS571, associated with Sesbania rostrata
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1 ORS571 Rhizobium sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Rhizobium sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.1 additional information
-
-
 Rhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Rhizobium sp. 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.6.1 dimer 2 * 36000, SDS-PAGE Rhizobium sp.
1.18.6.1 tetramer alpha: 56000, beta: 59000 Rhizobium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.6.1 30
-
 assay at Rhizobium sp.