BRENDA - Enzyme Database show

Purification aof nitrogenase from Clostridium pasteurianum

Mortenson, L.E.; Methods Enzymol. 24B, 446-456 (1972)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.18.6.1
Fe protein is salt sensitive
Clostridium pasteurianum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Clostridium pasteurianum
1.18.6.1
Molybdenum
-
Clostridium pasteurianum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
additional information
-
-
Clostridium pasteurianum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Clostridium pasteurianum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Clostridium pasteurianum
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.18.6.1
Fe protein is very O2 sensitive
Clostridium pasteurianum
Purification (Commentary)
EC Number
Commentary
Organism
1.18.6.1
strict anaerobic conditions
Clostridium pasteurianum
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Clostridium pasteurianum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
1.2
-
purified MoFe protein
Clostridium pasteurianum
1.18.6.1
1.7
2.2
purified Fe protein
Clostridium pasteurianum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440159
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440159
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440159
Clostridium pasteurianum
?
Subunits
EC Number
Subunits
Commentary
Organism
1.18.6.1
More
-
Clostridium pasteurianum
General Stability (protein specific)
EC Number
General Stability
Organism
1.18.6.1
Fe protein is salt sensitive
Clostridium pasteurianum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Clostridium pasteurianum
1.18.6.1
Molybdenum
-
Clostridium pasteurianum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
additional information
-
-
Clostridium pasteurianum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Clostridium pasteurianum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.18.6.1
Fe protein is very O2 sensitive
Clostridium pasteurianum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.6.1
strict anaerobic conditions
Clostridium pasteurianum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
1.2
-
purified MoFe protein
Clostridium pasteurianum
1.18.6.1
1.7
2.2
purified Fe protein
Clostridium pasteurianum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440159
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440159
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440159
Clostridium pasteurianum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.18.6.1
More
-
Clostridium pasteurianum