BRENDA - Enzyme Database show

Isolation and characterization of nitrogenase from Klebsiella pneumoniae

Shah, V.K.; Methods Enzymol. 118, 511-519 (1986)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Klebsiella pneumoniae
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Klebsiella pneumoniae
1.18.6.1
Molybdenum
-
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Klebsiella pneumoniae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.6.1
both components
Klebsiella pneumoniae
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Klebsiella pneumoniae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
2.082
-
purified component II
Klebsiella pneumoniae
1.18.6.1
2.16
-
purified component I
Klebsiella pneumoniae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440157
Klebsiella pneumoniae
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
440157
Klebsiella pneumoniae
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440157
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440157
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440157
Klebsiella pneumoniae
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440157
Klebsiella pneumoniae
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.18.6.1
30
-
assay at
Klebsiella pneumoniae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.18.6.1
7.4
-
assay at
Klebsiella pneumoniae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
-
Klebsiella pneumoniae
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Klebsiella pneumoniae
1.18.6.1
Molybdenum
-
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.6.1
both components
Klebsiella pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
2.082
-
purified component II
Klebsiella pneumoniae
1.18.6.1
2.16
-
purified component I
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
440157
Klebsiella pneumoniae
2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
440157
Klebsiella pneumoniae
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440157
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440157
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440157
Klebsiella pneumoniae
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440157
Klebsiella pneumoniae
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.18.6.1
30
-
assay at
Klebsiella pneumoniae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.18.6.1
7.4
-
assay at
Klebsiella pneumoniae