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Characterization of the metal clusters in the nitrogenase molybdenum-iron and vanadium-iron proteins of Azotobacter vinelandii using magnetic circular dichroism spectroscopy

Morningstar, J.E.; Johnson, M.K.; Case, E.E.; Hales, B.J.; Biochemistry 26, 1795-1800 (1987)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
characterization of the metal clusters in the nitrogenase molybdenum-iron and vanadium-iron proteins
Azotobacter vinelandii
1.18.6.1
Molybdenum
characterization of the metal clusters in the nitrogenase molybdenum-iron protein
Azotobacter vinelandii
1.18.6.1
Vanadium
characterization of the metal clusters in the nitrogenase vanadium-iron protein
Azotobacter vinelandii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Azotobacter vinelandii
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Azotobacter vinelandii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440156
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440156
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440156
Azotobacter vinelandii
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440156
Azotobacter vinelandii
?
-
-
-
?
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
characterization of the metal clusters in the nitrogenase molybdenum-iron and vanadium-iron proteins
Azotobacter vinelandii
1.18.6.1
Molybdenum
characterization of the metal clusters in the nitrogenase molybdenum-iron protein
Azotobacter vinelandii
1.18.6.1
Vanadium
characterization of the metal clusters in the nitrogenase vanadium-iron protein
Azotobacter vinelandii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440156
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440156
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440156
Azotobacter vinelandii
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440156
Azotobacter vinelandii
?
-
-
-
?