Literature summary extracted from
Pau, R.N.; Mitchenall, L.A.; Robson, R.L.
Genetic evidence for an Azotobacter vinelandii nitrogenase lacking molybdenum and vanadium (1989), J. Bacteriol., 171, 124-129.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.18.6.1 |
genetic analysis |
Azotobacter vinelandii |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.18.6.1 |
additional information |
construction of mutant strain RP114 |
Azotobacter vinelandii |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.18.6.1 |
Molybdenum |
also possesses Mo-independent nitrogenases: one vanadium containing nitrogenase and another lacking both molybdenum and vanadium |
Azotobacter vinelandii |
|
1.18.6.1 |
Vanadium |
possesses 2 molybdenum-independent nitrogenases: one vanadium-containing nitrogenase and another lacking both molybdenum and vanadium |
Azotobacter vinelandii |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.18.6.1 |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O |
Azotobacter vinelandii |
- |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.18.6.1 |
Azotobacter vinelandii |
- |
contains 3 classes of nitrogenase, the second contains V, the third is encoded by a separate set of genes and is lacking V and Mo and is inhibited by V and Mo |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.18.6.1 |
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate |
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein |
Azotobacter vinelandii |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.18.6.1 |
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O |
- |
Azotobacter vinelandii |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate |
- |
? |
|