BRENDA - Enzyme Database show

Molybdenum in nitrogenase

Shah, V.K.; Ugalde, R.A.; Imperial, J.; Brill, W.J.; Annu. Rev. Biochem. 53, 231-257 (1984)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.18.6.1
-
Azotobacter vinelandii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.18.6.1
CO
-
Clostridium pasteurianum
1.18.6.1
CO
-
Klebsiella pneumoniae
1.18.6.1
CO
-
Paenibacillus polymyxa
1.18.6.1
CO
-
Azotobacter vinelandii
1.18.6.1
O2
-
Clostridium pasteurianum
1.18.6.1
O2
-
Paenibacillus polymyxa
1.18.6.1
O2
-
Klebsiella pneumoniae
1.18.6.1
O2
-
Azotobacter vinelandii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
4 atoms of iron per molecule of Fe protein; enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
Azotobacter vinelandii
1.18.6.1
Iron
4 atoms of iron per molecule of Fe protein; enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
Klebsiella pneumoniae
1.18.6.1
Iron
-
Paenibacillus polymyxa
1.18.6.1
Iron
-
Clostridium pasteurianum
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Paenibacillus polymyxa
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Klebsiella pneumoniae
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Clostridium pasteurianum
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Azotobacter vinelandii
1.18.6.1
Molybdenum
enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex; molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview; review on molybdenum in nitrogenase
Azotobacter vinelandii
1.18.6.1
Molybdenum
molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview; review on molybdenum in nitrogenase
Clostridium pasteurianum
1.18.6.1
Molybdenum
6 iron atoms to 1 molybdenum atom in MoFe protein; enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex; molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview; review on molybdenum in nitrogenase
Klebsiella pneumoniae
1.18.6.1
Molybdenum
review on molybdenum in nitrogenase
Paenibacillus polymyxa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
55000
60000
component II Fe protein
Azotobacter vinelandii
1.18.6.1
55000
60000
component II Fe protein
Clostridium pasteurianum
1.18.6.1
55000
60000
component II Fe protein
Klebsiella pneumoniae
1.18.6.1
220000
250000
component I MoFe protein
Azotobacter vinelandii
1.18.6.1
220000
250000
component I MoFe protein
Clostridium pasteurianum
1.18.6.1
220000
250000
component I MoFe protein
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Clostridium pasteurianum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Paenibacillus polymyxa
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Azotobacter vinelandii
-
-
-
1.18.6.1
Clostridium pasteurianum
-
-
-
1.18.6.1
Klebsiella pneumoniae
-
nitrogen fixation complex is encoded on nif gene cluster
-
1.18.6.1
Paenibacillus polymyxa
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.18.6.1
MoFe protein is extremely sensitive to O2
Klebsiella pneumoniae
1.18.6.1
MoFe protein is extremely sensitive to O2
Azotobacter vinelandii
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
catalytic mechanism; enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Azotobacter vinelandii
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
catalytic mechanism; enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Clostridium pasteurianum
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
catalytic mechanism; enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Paenibacillus polymyxa
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
catalytic mechanism; enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Klebsiella pneumoniae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Klebsiella pneumoniae
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Clostridium pasteurianum
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Azotobacter vinelandii
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Paenibacillus polymyxa
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Paenibacillus polymyxa
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Paenibacillus polymyxa
?
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.18.6.1
-
Azotobacter vinelandii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.18.6.1
CO
-
Clostridium pasteurianum
1.18.6.1
CO
-
Klebsiella pneumoniae
1.18.6.1
CO
-
Paenibacillus polymyxa
1.18.6.1
CO
-
Azotobacter vinelandii
1.18.6.1
O2
-
Clostridium pasteurianum
1.18.6.1
O2
-
Paenibacillus polymyxa
1.18.6.1
O2
-
Klebsiella pneumoniae
1.18.6.1
O2
-
Azotobacter vinelandii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.18.6.1
Iron
4 atoms of iron per molecule of Fe protein; enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
Azotobacter vinelandii
1.18.6.1
Iron
4 atoms of iron per molecule of Fe protein; enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex
Klebsiella pneumoniae
1.18.6.1
Iron
-
Paenibacillus polymyxa
1.18.6.1
Iron
-
Clostridium pasteurianum
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Paenibacillus polymyxa
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Klebsiella pneumoniae
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Clostridium pasteurianum
1.18.6.1
Mg2+
Mg2+ required for MgATP complex
Azotobacter vinelandii
1.18.6.1
Molybdenum
enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex; molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview; review on molybdenum in nitrogenase
Azotobacter vinelandii
1.18.6.1
Molybdenum
molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview; review on molybdenum in nitrogenase
Clostridium pasteurianum
1.18.6.1
Molybdenum
6 iron atoms to 1 molybdenum atom in MoFe protein; enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex; molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview; review on molybdenum in nitrogenase
Klebsiella pneumoniae
1.18.6.1
Molybdenum
review on molybdenum in nitrogenase
Paenibacillus polymyxa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.6.1
55000
60000
component II Fe protein
Azotobacter vinelandii
1.18.6.1
55000
60000
component II Fe protein
Clostridium pasteurianum
1.18.6.1
55000
60000
component II Fe protein
Klebsiella pneumoniae
1.18.6.1
220000
250000
component I MoFe protein
Azotobacter vinelandii
1.18.6.1
220000
250000
component I MoFe protein
Clostridium pasteurianum
1.18.6.1
220000
250000
component I MoFe protein
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Clostridium pasteurianum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Azotobacter vinelandii
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Paenibacillus polymyxa
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.18.6.1
MoFe protein is extremely sensitive to O2
Klebsiella pneumoniae
1.18.6.1
MoFe protein is extremely sensitive to O2
Azotobacter vinelandii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Klebsiella pneumoniae
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Clostridium pasteurianum
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Clostridium pasteurianum
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Azotobacter vinelandii
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Azotobacter vinelandii
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Paenibacillus polymyxa
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440147
Paenibacillus polymyxa
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440147
Paenibacillus polymyxa
?