BRENDA - Enzyme Database show

Oxygen inhibition of nitrogenase activity in Klebsiella pneumoniae

Kavanagh, E.P.; Hill, S.; J. Gen. Microbiol. 139, 1307-1314 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.18.6.1
O2
complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2
Klebsiella pneumoniae
1.19.6.1
O2
complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2
Klebsiella pneumoniae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.19.6.1
Mg2+
required for ATP hydrolysis
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.19.6.1
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
Klebsiella pneumoniae
anaerobic conditions are required by the enzyme
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.6.1
Klebsiella pneumoniae
-
-
-
1.19.6.1
Klebsiella pneumoniae
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.18.6.1
complete reversible inhibition by O2, reversibility decreases by increasing the time of exposure to O2, after 20 min 60% reversibility of the inhibition remains
Klebsiella pneumoniae
1.19.6.1
complete reversible inhibition by O2, reversibility decreases by increasing the time of exposure to O2, after 20 min 60% reversibility of the inhibition remains
Klebsiella pneumoniae
Reaction
EC Number
Reaction
Commentary
Organism
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein
Klebsiella pneumoniae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
0.027
-
reductant dithionite, whole cell assay
Klebsiella pneumoniae
1.19.6.1
0.027
-
reductant dithionite
Klebsiella pneumoniae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440140
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440140
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440140
Klebsiella pneumoniae
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440140
Klebsiella pneumoniae
?
-
-
-
?
1.19.6.1
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
440140
Klebsiella pneumoniae
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
440140
Klebsiella pneumoniae
?
1.19.6.1
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
anaerobic conditions are required by the enzyme
440140
Klebsiella pneumoniae
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
-
-
?
1.19.6.1
dithionite + H+ + N2 + ATP
-
440140
Klebsiella pneumoniae
?
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.18.6.1
O2
complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2
Klebsiella pneumoniae
1.19.6.1
O2
complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2
Klebsiella pneumoniae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.19.6.1
Mg2+
required for ATP hydrolysis
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
?
1.19.6.1
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
Klebsiella pneumoniae
anaerobic conditions are required by the enzyme
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
-
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.18.6.1
complete reversible inhibition by O2, reversibility decreases by increasing the time of exposure to O2, after 20 min 60% reversibility of the inhibition remains
Klebsiella pneumoniae
1.19.6.1
complete reversible inhibition by O2, reversibility decreases by increasing the time of exposure to O2, after 20 min 60% reversibility of the inhibition remains
Klebsiella pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.6.1
0.027
-
reductant dithionite, whole cell assay
Klebsiella pneumoniae
1.19.6.1
0.027
-
reductant dithionite
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440140
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
-
-
?
1.18.6.1
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
440140
Klebsiella pneumoniae
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
440140
Klebsiella pneumoniae
?
1.18.6.1
dithionite + H+ + N2 + ATP
in vitro substrate
440140
Klebsiella pneumoniae
?
-
-
-
?
1.19.6.1
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
440140
Klebsiella pneumoniae
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
440140
Klebsiella pneumoniae
?
1.19.6.1
6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
anaerobic conditions are required by the enzyme
440140
Klebsiella pneumoniae
6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
-
-
?
1.19.6.1
dithionite + H+ + N2 + ATP
-
440140
Klebsiella pneumoniae
?
-
-
-
?