Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kavanagh, E.P.; Hill, S.
    Oxygen inhibition of nitrogenase activity in Klebsiella pneumoniae (1992), J. Gen. Microbiol., 139, 1307-1314.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.6.1 O2 complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2 Klebsiella pneumoniae
1.19.6.1 O2 complete reversible inhibition, reversibility decreases by increasing the time of exposure to O2 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.19.6.1 Mg2+ required for ATP hydrolysis Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
1.19.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP Klebsiella pneumoniae anaerobic conditions are required by the enzyme 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Klebsiella pneumoniae
-
-
-
1.19.6.1 Klebsiella pneumoniae
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.18.6.1 complete reversible inhibition by O2, reversibility decreases by increasing the time of exposure to O2, after 20 min 60% reversibility of the inhibition remains Klebsiella pneumoniae
1.19.6.1 complete reversible inhibition by O2, reversibility decreases by increasing the time of exposure to O2, after 20 min 60% reversibility of the inhibition remains Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.1 0.027
-
reductant dithionite, whole cell assay Klebsiella pneumoniae
1.19.6.1 0.027
-
reductant dithionite Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?
1.18.6.1 dithionite + H+ + N2 + ATP in vitro substrate Klebsiella pneumoniae ?
-
?
1.19.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP
-
Klebsiella pneumoniae 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
?
1.19.6.1 6 reduced flavodoxin + N2 + 6 H2O + 6 ATP anaerobic conditions are required by the enzyme Klebsiella pneumoniae 6 oxidized flavodoxin + 2 NH3 + 6 H+ + 6 ADP + 6 phosphate
-
?
1.19.6.1 dithionite + H+ + N2 + ATP
-
Klebsiella pneumoniae ?
-
?