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Literature summary extracted from

  • Takeyama, K.i.; Kitanaka, S.; Sato, T.; Kobori, M.; Yanagisawa, J.; Kato, S.
    25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis (1997), Science, 277, 1827-1830.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.18 expression in COS cells, reconstituted with recombinant cytochrome P-450, recombinant adrenodoxin, and recombinant adrenodoxin reductase Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.18 additional information construction and expression in COS cell line of a chimeric fusion protein: vitaminD receptor ligand-binding domain with the yeast GAL4 DNA-binding domain, also expression of lacZ containing reporter plasmid Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.18 1,25-Dihydroxycholecalciferol calcitriol; in vivo only inhibitory in vitamin D receptor containing cells, therefore: negative feedback inhibition is mediated by the enzyme through liganded vitamin D receptor Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.18 55000
-
 SDS-PAGE after in vitro-translation Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.18 Mus musculus O35084
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O 25-hydroxyvitamin D3 1alpha-hydroxylase is a cytochrome P-450-dependent mixed-function oxidase Mus musculus
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O amino acid sequence determination Mus musculus
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.18 kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Mus musculus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.18 NADPH
-
 Mus musculus