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Literature summary extracted from

  • Henry, H.L.; Norman, A.W.
    Studies on calciferol metabolism. IX. Renal 25-hydroxy-vitamin D3-1 hydroxylase. Involvement of cytochrome P-450 and other properties (1974), J. Biol. Chem., 249, 7529-7535.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.18 CN- activates with malate as electron donor Gallus gallus
1.14.15.18 malate supports 1alpha-hydroxylation as electron donor Gallus gallus
1.14.15.18 succinate supports 1alpha-hydroxylation as electron donor Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.18 antimycin A higher inhibition rate with succinate as electron donor than with malate Gallus gallus
1.14.15.18 Ca2+
-
 Gallus gallus
1.14.15.18 dinitrophenol higher inhibition rate with malate as electron donor than with succinate Gallus gallus
1.14.15.18 Mn2+
-
 Gallus gallus
1.14.15.18 additional information no inhibition with Ba2+, acetate, sulfate Gallus gallus
1.14.15.18 phosphate
-
 Gallus gallus
1.14.15.18 Sr2+
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.18 additional information
-
 additional information
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.18 mitochondrion
-
 Gallus gallus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Gallus gallus
-
 calcitriol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.18 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.18 kidney
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2
-
 Gallus gallus calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Gallus gallus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.18 NADPH
-
 Gallus gallus