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Literature summary extracted from

  • Becker, H.D.; Roy, H.; Moulinier, L.; Mazauric, M.H.; Keith, G.; Kern, D.
    Thermus thermophilus contains an eubacterial and an archaebacterial aspartyl-tRNA synthetase (2000), Biochemistry, 39, 3216-3230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.12 aspS1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21 Thermus thermophilus
6.1.1.12 aspS2 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha and BL21 Thermus thermophilus
6.1.1.23 overexpression in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.12 0.00003
-
 tRNAAsp pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.000063
-
 tRNAAsn pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.000073
-
 tRNAAsp pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.0034
-
 tRNAAsn pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.005
-
 L-aspartic acid pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.03
-
 aspartic acid pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.033
-
 ATP pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.28
-
 ATP pH 7.2, 70°C Thermus thermophilus
6.1.1.23 0.00003
-
 tRNAAsp AspRS1 Thermus thermophilus
6.1.1.23 0.00006
-
 tRNAAsn AspRS2 Thermus thermophilus
6.1.1.23 0.00007
-
 tRNAAsp AspRS2 Thermus thermophilus
6.1.1.23 0.005
-
 aspartate AspRS2 Thermus thermophilus
6.1.1.23 0.03
-
 aspartate AspRS1 Thermus thermophilus
6.1.1.23 0.033
-
 ATP AspRS2 Thermus thermophilus
6.1.1.23 0.28
-
 ATP AspRS1 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.12 48329
-
 x * 48329, amino acid sequence determination Thermus thermophilus
6.1.1.12 66029
-
 x * 66029, amino acid sequence determination Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermus thermophilus
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp Thermus thermophilus
-
 AMP + diphosphate + Asp-tRNAAsp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.12 Thermus thermophilus P36419 Asp-tRNA synthetase AspRS1
-
6.1.1.12 Thermus thermophilus Q5SIC2 Asp-tRNA synthetase AspRS2
-
6.1.1.12 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 P36419 Asp-tRNA synthetase AspRS1
-
6.1.1.12 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SIC2 Asp-tRNA synthetase AspRS2
-
6.1.1.23 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.12 recombinant from overexpressing Escherichia coli, 34.3fold Thermus thermophilus
6.1.1.12 recombinant from overexpressing Escherichia coli, 7.4fold Thermus thermophilus
6.1.1.23 recombinant enzyme Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.12 ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp conserved amino acid residues of functional importance in consensus motifs: Pro227 in motif 1, Arg332 and Glu334 in motif 2, Gly675, Gly679, Asp681, Arg682, and Pro700 in motif 3 Thermus thermophilus
a
6.1.1.12 ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp conserved amino acid residues of functional importance in consensus motifs: Pro227 in motif 1, Arg332 and Glu334 in motif 2, Gly675, Gly679, Glu681, Arg682, and Pro700 in motif 3 Thermus thermophilus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.12 0.00002
-
 native enzyme, cell extract, 37°C Thermus thermophilus
6.1.1.12 0.000083
-
 native enzyme, cell extract, 37°C Thermus thermophilus
6.1.1.12 0.0336
-
 purified recombinant enzyme, 37°C Thermus thermophilus
6.1.1.12 0.043
-
 recombinant enzyme, Escherichia coli DH5alpha cell extract, 37°C Thermus thermophilus
6.1.1.12 0.055
-
 recombinant enzyme, Escherichia coli BL21 cell extract, 37°C Thermus thermophilus
6.1.1.12 0.124
-
 recombinant enzyme, Escherichia coli BL21 cell extract, 37°C Thermus thermophilus
6.1.1.12 0.155
-
 recombinant enzyme, Escherichia coli DH5alpha cell extract, 37°C Thermus thermophilus
6.1.1.12 0.321
-
 purified recombinant enzyme, 37°C Thermus thermophilus
6.1.1.23 32.1
-
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.12 ATP + L-aspartate + tRNAAsn about half as effective as tRNAAsp Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsn about have as effective as tRNAAsp Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsn about half as effective as tRNAAsp Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsn about have as effective as tRNAAsp Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermus thermophilus or Escherichia coli tRNA Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermus thermophilus or Eschrichia coli tRNA Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermus thermophilus or Escherichia coli tRNA Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp Thermus thermophilus or Eschrichia coli tRNA Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn only the enzyme AspRS2 aspartylates tRNAAsn Thermus thermophilus AMP + diphosphate + Asp-tRNAAsn
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
 Thermus thermophilus AMP + diphosphate + Asp-tRNAAsp
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.12 ? x * 48329, amino acid sequence determination Thermus thermophilus
6.1.1.12 ? x * 66029, amino acid sequence determination Thermus thermophilus
6.1.1.12 More modular organization Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.12 Aspartic acid translase
-
 Thermus thermophilus
6.1.1.12 Aspartyl ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.12 aspartyl ribonuleic synthetase
-
 Thermus thermophilus
6.1.1.12 Aspartyl-transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.12 Aspartyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.12 Aspartyl-tRNA synthetase
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.12 0.092
-
 tRNAAsn pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.12
-
 tRNAAsn pH 7.2, 70°C Thermus thermophilus
6.1.1.12 0.24
-
 tRNAAsp pH 7.2, 70°C Thermus thermophilus
6.1.1.12 2.7
-
 tRNAAsp pH 7.2, 70°C Thermus thermophilus
6.1.1.23 0.092
-
 tRNAAsn AspRS2 Thermus thermophilus
6.1.1.23 0.24
-
 tRNAAsp AspRS2 Thermus thermophilus
6.1.1.23 7.2
-
 tRNAAsp AspRS1 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.12 7.2
-
 assay at Thermus thermophilus