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Literature summary extracted from

  • Zhang, J.; Wolfe, S.; Demain, A.L.
    Biochemical studies on the activity of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus (1992), Biochem. J., 283, 691-698.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.26 5,5'-dithiobis-2-nitrobenzoate 1 mM Streptomyces clavuligerus
6.3.2.26 AMP 5 mM Streptomyces clavuligerus
6.3.2.26 ATP at concentrations about 5 mM Streptomyces clavuligerus
6.3.2.26 diphosphate 5 mM Streptomyces clavuligerus
6.3.2.26 EDTA 10 mM Streptomyces clavuligerus
6.3.2.26 iodoacetamide 1 mM Streptomyces clavuligerus
6.3.2.26 N-ethylmaleimide 1 mM Streptomyces clavuligerus
6.3.2.26 pyridoxal 5'-phosphate 2 mM Streptomyces clavuligerus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.26 0.12
-
L-cysteine
-
Streptomyces clavuligerus
6.3.2.26 0.3
-
L-valine
-
Streptomyces clavuligerus
6.3.2.26 0.63
-
L-aminoadipate
-
Streptomyces clavuligerus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.26 Mg2+ optimal concentration: 10 mM Streptomyces clavuligerus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
6.3.2.26 dithiothreitol 8 mM leads to a 10% higher enzyme activity than at 3 mM Streptomyces clavuligerus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.26 Streptomyces clavuligerus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.26 0.0113
-
-
Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.26 L-2-aminoadipate + L-cysteine + L-allo-isoleucine + ATP
-
Streptomyces clavuligerus L-delta-(aminoadipyl)-L-cysteinyl-D-allo-isoleucine + AMP + diphosphate
-
?
6.3.2.26 L-2-aminoadipate + L-cysteine + L-isoleucine + ATP
-
Streptomyces clavuligerus L-delta-(aminoadipyl)-L-cysteinyl-D-isoleucine + AMP + diphosphate
-
?
6.3.2.26 L-2-aminoadipate + L-cysteine + L-valine + ATP
-
Streptomyces clavuligerus N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + AMP + diphosphate
-
ir
6.3.2.26 L-2-aminoadipate + L-homocysteine + L-valine + ATP
-
Streptomyces clavuligerus N-(5-amino-5-carboxypentanoyl)-L-homocysteinyl-D-valine + AMP + diphosphate
-
?
6.3.2.26 L-glutamate + L-cysteine + L-valine + ATP
-
Streptomyces clavuligerus L-glutamyl-L-cysteinyl-D-valine + AMP + diphosphate
-
?
6.3.2.26 S-carboxymethylcysteine + L-cysteine + L-valine + ATP
-
Streptomyces clavuligerus L-S-carboxymethylcysteinyl-L-cysteinyl-D-valine + AMP + diphosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.26 29.5
-
-
Streptomyces clavuligerus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.2.26 4 32
-
Streptomyces clavuligerus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.26 4
-
half of activity after 70 h Streptomyces clavuligerus
6.3.2.26 25
-
half of activity after 10 h Streptomyces clavuligerus
6.3.2.26 32
-
half of activity after 10 min Streptomyces clavuligerus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.26 8 8.5
-
Streptomyces clavuligerus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.26 7 9
-
Streptomyces clavuligerus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.2.26 7
-
very unstable below pH 7 Streptomyces clavuligerus
6.3.2.26 8.5
-
very unstable above pH 8.5 Streptomyces clavuligerus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.26 ATP optimal concentration: 1.5-5 mM Streptomyces clavuligerus