Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Peters, R.J.; Carter, O.A.; Zhang, Y.; Matthews, B.W.; Croteau, R.B.
    Bifunctional abietadiene synthase: mutual structural dependence of the active sites for protonation-initiated and ionization-initiated cyclizations (2003), Biochemistry, 42, 2700-2707.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.12 expression in Escherichia coli Abies grandis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.18 DELTA:107-868 lower turnover with copalyl diphosphate than wild-type Abies grandis
4.2.3.18 DELTA:85-849 no turnover with copalyl diphosphate Abies grandis
4.2.3.18 rAS:D96A nearly the same turnover with copalyl diphosphate like wild-type Abies grandis
4.2.3.18 rAS:K86A/R87A lower turnover with copalyl diphosphate than wild-type Abies grandis
5.5.1.12 DELTA:107-868 lower turnover with copalyl diphosphate than wild-type Abies grandis
5.5.1.12 DELTA:85-849 no turnover with copalyl diphosphate Abies grandis
5.5.1.12 rAS:D96A nearly the same turnover with copalyl diphosphate like wild-type Abies grandis
5.5.1.12 rAS:K86A/R87A lower turnover with copalyl diphosphate than wild-type Abies grandis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.18 0.0005
-
 copalyl diphosphate mutants rAS:K86A/R87A Abies grandis
4.2.3.18 0.0007
-
 copalyl diphosphate mutants ASDELTA:107-868 Abies grandis
4.2.3.18 0.0009
-
 copalyl diphosphate mutant rAS:D96A Abies grandis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.18 (+)-copalyl-diphosphate Abies grandis
-
 (-)-abietadiene + diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate Abies grandis
-
 (+)-copalyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.18 Abies grandis
-
-
-
5.5.1.12 Abies grandis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.18 (+)-copalyl-diphosphate
-
 Abies grandis (-)-abietadiene + diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate
-
 Abies grandis (+)-copalyl diphosphate
-
 ?