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Literature summary extracted from

  • Kunugi, S.; Uehara-Kunugi, Y.; v.d.Haar, F.; Schischkoff, J.; Freist, W.; Englisch, U.; Cramer, F.
    Biochemical comparison of the Neurospora crassa wild type and the temperature-sensitive and leucine-auxotroph mutant leu-5 (1986), Eur. J. Biochem., 158, 43-49.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 additional information temperature-sensitive and leucine-auxotroph mutant leu-5 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 1
-
 8-bromoadenosine 5'-triphosphate 3'-dATP, tubercidin triphosphate Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Neurospora crassa
-
 wild-type and temperature-sensitive and leucine-auxotroph mutant leu-5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 wild-type enzyme, mutant enzyme found only in traces Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.4 0.325
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 3'-dATP + L-leucine + tRNALeu
-
 Neurospora crassa 3'-dAMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 8-bromoadenosine 5'-triphosphate + L-leucine + tRNALeu
-
 Neurospora crassa 8-bromoadenosine 5'-monophosphate + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-isoleucine + tRNALeu
-
 Neurospora crassa AMP + diphosphate + L-isoleucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Neurospora crassa AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information leucine-dependent ATP-diphosphate exchange, leucine + ATP + enzyme/Ile-AMP-enzyme + diphosphate Neurospora crassa ?
-
 ?
6.1.1.4 tubercidin 5'-triphosphate + L-leucine + tRNALeu
-
 Neurospora crassa tubercidin 5'-phosphate + diphosphate + L-leucyl-tRNALeu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 2 3.7 Leu aminoacylation Neurospora crassa
6.1.1.4 20 50 Leu ATP-diphosphate exchange reaction Neurospora crassa