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Literature summary extracted from

  • Yu, L.; Mei, Q.C.; Yu, C.A.
    Characterization of purified cytochrome b-c1 complex from Rhodopseudomonas sphaeroides R-26 (1984), J. Biol. Chem., 259, 5752-5760.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.8 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole
-
Bos taurus
7.1.1.8 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole
-
Cereibacter sphaeroides
7.1.1.8 antimycin A
-
Bos taurus
7.1.1.8 antimycin A
-
Cereibacter sphaeroides
7.1.1.8 antimycin A
-
Spinacia oleracea
7.1.1.8 additional information insensitive to N',N'-dicyclohexylcarbodiimide Cereibacter sphaeroides
7.1.1.8 Phospholipase A2
-
Cereibacter sphaeroides
7.1.1.8 undecyl-hydroxy-dioxobenzoxythiazole
-
Bos taurus
7.1.1.8 undecyl-hydroxy-dioxobenzoxythiazole
-
Cereibacter sphaeroides
7.1.1.8 undecyl-hydroxy-dioxobenzoxythiazole
-
Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.8 0.004
-
ubiquinol-10
-
Cereibacter sphaeroides
7.1.1.8 0.006
-
ubiquinol-10
-
Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.8 chromatophore
-
Cereibacter sphaeroides
-
-
7.1.1.8 mitochondrion
-
Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.8 Iron-sulfur cluster
-
Bos taurus
7.1.1.8 Iron-sulfur cluster
-
Cereibacter sphaeroides
7.1.1.8 Iron-sulfur cluster
-
Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.8 30000
-
cytochrome c, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 48000
-
cytochrome b, SDS-PAGE Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.8 ubiquinone-10 + ferricytochrome c Bos taurus
-
ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c Cereibacter sphaeroides
-
ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c Spinacia oleracea
-
ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c Cereibacter sphaeroides GA
-
ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c Cereibacter sphaeroides R-26
-
ubiquinol-10 + ferrocytochrome c + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.8 Bos taurus
-
-
-
7.1.1.8 Cereibacter sphaeroides
-
-
-
7.1.1.8 Cereibacter sphaeroides
-
formerly Rhodopseudomonas sphaeroides
-
7.1.1.8 Cereibacter sphaeroides
-
blue-green mutant
-
7.1.1.8 Cereibacter sphaeroides GA
-
-
-
7.1.1.8 Cereibacter sphaeroides R-26
-
blue-green mutant
-
7.1.1.8 Spinacia oleracea
-
spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.8
-
Cereibacter sphaeroides
7.1.1.8
-
Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
7.1.1.8 -70°C, stable for several weeks Cereibacter sphaeroides
7.1.1.8 -80°C, lyophilized in 50% v/v glycerol Cereibacter sphaeroides
7.1.1.8 0°C, stable for several hours Cereibacter sphaeroides
7.1.1.8 20°C loses 13% of its activity after 1 h Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.8 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1
-
Cereibacter sphaeroides 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+[side 2]
-
?
7.1.1.8 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1
-
Cereibacter sphaeroides GA 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+[side 2]
-
?
7.1.1.8 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1
-
Cereibacter sphaeroides R-26 2-azido-3-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+[side 2]
-
?
7.1.1.8 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1
-
Cereibacter sphaeroides 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+
-
?
7.1.1.8 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1
-
Cereibacter sphaeroides GA 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+
-
?
7.1.1.8 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinone + 2 ferricytochrome c1
-
Cereibacter sphaeroides R-26 3-azido-2-methyl-5-methoxy-6-geranyl-1,4-benzoquinol + 2 ferrocytochrome c1 + 2 H+
-
?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
Bos taurus ubiquinone + 2 ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
Cereibacter sphaeroides ubiquinone + 2 ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
Spinacia oleracea ubiquinone + 2 ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
Cereibacter sphaeroides GA ubiquinone + 2 ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
Cereibacter sphaeroides R-26 ubiquinone + 2 ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinol 10 + cytochrome c
-
Bos taurus ubiquinone 10 + reduced cytochrome c
-
?
7.1.1.8 ubiquinol 10 + cytochrome c
-
Cereibacter sphaeroides ubiquinone 10 + reduced cytochrome c
-
?
7.1.1.8 ubiquinol 10 + cytochrome c
-
Cereibacter sphaeroides GA ubiquinone 10 + reduced cytochrome c
-
?
7.1.1.8 ubiquinol 10 + cytochrome c
-
Cereibacter sphaeroides R-26 ubiquinone 10 + reduced cytochrome c
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
Bos taurus ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
Cereibacter sphaeroides ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
Spinacia oleracea ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
Cereibacter sphaeroides GA ubiquinol-10 + ferrocytochrome c + 2 H+
-
?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
Cereibacter sphaeroides R-26 ubiquinol-10 + ferrocytochrome c + 2 H+
-
?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.8 b6/f complex
-
Spinacia oleracea
7.1.1.8 cytochrome b-c1 complex
-
Cereibacter sphaeroides
7.1.1.8 cytochrome b-c2 complex
-
Cereibacter sphaeroides
7.1.1.8 plastoquinol-plastocyanin oxidoreductase
-
Spinacia oleracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.1.1.8
-
-
stable for several hours Cereibacter sphaeroides
7.1.1.8 37
-
stable at lower temperatures, becomes less stable when incubation temperature is raised, half-life time at 37°C is 15 min Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.1.1.8 5 9.5
-
Cereibacter sphaeroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
7.1.1.8 5 9.5 irreversibly denatured at pH lower than 5.0, stable at neutral pH, at pH 9.5 40% of the enzyme is irreversibly denatured after 19h Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.8 cytochrome b
-
Bos taurus
7.1.1.8 cytochrome b
-
Cereibacter sphaeroides
7.1.1.8 cytochrome c1
-
Bos taurus
7.1.1.8 cytochrome c1
-
Cereibacter sphaeroides
7.1.1.8 Phospholipid
-
Bos taurus
7.1.1.8 Phospholipid
-
Cereibacter sphaeroides
7.1.1.8 ubiquinone
-
Bos taurus
7.1.1.8 ubiquinone
-
Cereibacter sphaeroides