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Literature summary extracted from

  • Nelson, B.D.; Gellerfors, P.
    Characterization and resolution of complex III from beef heart mitochondria (1978), Methods Enzymol., 53, 80-91.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.8 Antimycin
-
 Bos taurus
7.1.1.8 Antimycin
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.8 mitochondrion
-
 Saccharomyces cerevisiae 5739
-
7.1.1.8 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.8 10000
-
 1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE Bos taurus
7.1.1.8 13000
-
 1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE Bos taurus
7.1.1.8 15000
-
 1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE Bos taurus
7.1.1.8 26000
-
 iron-sulfur protein, SDS-PAGE Bos taurus
7.1.1.8 29000
-
 cytochrome c1, SDS-PAGE Bos taurus
7.1.1.8 47000
-
 core protein 2, SDS-PAGE Bos taurus
7.1.1.8 50000
-
 core protein 1, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.8 Bos taurus
-
-
-
7.1.1.8 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.8
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.8 heart
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
7.1.1.8 -20°C, isolated complex III is stored in presence of 50% glycerol, can be used for functional studies up to a month, for preparation of subunits only freshly prepared bc1 complex, because storage leads to partial degradation of some subunits by proteases Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.8 tetramethyl-p-benzoquinol + cytochrome c duroquinol Bos taurus tetramethyl-p-benzoquinone + reduced cytochrome c
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Saccharomyces cerevisiae ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Bos taurus ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.8 heptamer
-
 Saccharomyces cerevisiae
7.1.1.8 heptamer 1 * 50000 + 1 * 47000 + 1 * 29000 + 1 * 26000 + 1 * 15000 + 1 * 13000 + 1 * 10000, SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.8 cytochrome b-562
-
 Bos taurus
7.1.1.8 cytochrome b-565
-
 Bos taurus