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Literature summary extracted from
- Purification and characterisation of ascorbate oxidase from flour and immature wheat kernels. (1999), J. Cereal Sci., 30, 245-254.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.3 | 0.3 | - |
L-ascorbate | - |
Triticum aestivum |  |
| 1.10.3.3 | 0.53 | - |
D-isoascorbate | - |
Triticum aestivum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.3 | 72000 | - |
2 * 72000, basic enzyme, SDS-PAGE | Triticum aestivum |
| 1.10.3.3 | 139000 | - |
basic enzyme, gel filtration | Triticum aestivum |
| 1.10.3.3 | 140000 | - |
acidic enzyme, gel filtration | Triticum aestivum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.3 | Triticum aestivum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.3 | ammonium sulfate, phenyl Sepharose, Sephacryl S-300, Q-Sepharose, preparative isoelectric focusing, basic and acidic enzyme purified from flour, ammonium sulfate, phenyl Sepharose, Q-Sepharose, Mono S, Superdex 200, basic and acidic enzyme purified from immature kernels | Triticum aestivum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.3 | 2 L-ascorbate + O2 | - |
Triticum aestivum | 2 L-dehydroascorbate + 2 H2O | - |
? | |
| 1.10.3.3 | D-isoascorbate + O2 | - |
Triticum aestivum | 2-dehydroisoascorbate + H2O | - |
r | |
| 1.10.3.3 | D-isoascorbic acid + O2 | - |
Triticum aestivum | 2-dehydroisoascorbate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.10.3.3 | dimer | 2 * 72000, basic enzyme, SDS-PAGE | Triticum aestivum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.3 | 50 | - |
- |
Triticum aestivum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.3 | 40 | - |
stable for 30 min | Triticum aestivum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.3 | 6.2 | - |
basic enzyme | Triticum aestivum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.3 | 5 | 9 | - |
Triticum aestivum |
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Release 2023.1 (January 2023)
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