EC Number | Application | Comment | Organism |
---|---|---|---|
1.10.3.3 | analysis | quantitative determination of ascorbic acid with enzyme elctrode containing enzyme immobilized on nylon membrane | Cucurbita sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.3 | 0.37 | - |
chlorohydroquinone | - |
Cucurbita sp. | |
1.10.3.3 | 1.5 | - |
L-ascorbic acid | - |
Cucurbita sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.3 | copper | - |
Cucurbita sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.3 | Cucurbita sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.3 | ammonium sulfate, DEAE-dextran-silochrome | Cucurbita sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.10.3.3 | fruit | - |
Cucurbita sp. | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.3 | 2 L-ascorbate + O2 | - |
Cucurbita sp. | 2 L-dehydroascorbate + 2 H2O | - |
? | |
1.10.3.3 | chlorohydroquinone + O2 | 6% of activity with L-ascorbic acid | Cucurbita sp. | chloroquinone + H2O | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.3 | 55 | - |
no loss in activity after 30 min, approx. 55% activity after 30 min at 60°C, approx. 30% activity after 30 min at 65°C | Cucurbita sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.3 | 6.5 | - |
- |
Cucurbita sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.3 | 5 | 10 | rapid loss of activity below pH 4 and above pH 12 | Cucurbita sp. |