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Literature summary extracted from

  • Sereikaite, J.; Iljaseviciene, D.; Dienys, G.; Danilcenko, H.; Gavrilova, V.
    Ascorbate oxidase: Specificity and analytical application (1993), Appl. Biochem. Biotechnol., 43, 153-160.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.3 analysis quantitative determination of ascorbic acid with enzyme elctrode containing enzyme immobilized on nylon membrane Cucurbita sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.3 0.37
-
 chlorohydroquinone
-
 Cucurbita sp.
1.10.3.3 1.5
-
 L-ascorbic acid
-
 Cucurbita sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.3 copper
-
 Cucurbita sp.

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.3 Cucurbita sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.3 ammonium sulfate, DEAE-dextran-silochrome Cucurbita sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.3 fruit
-
 Cucurbita sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.3 2 L-ascorbate + O2
-
 Cucurbita sp. 2 L-dehydroascorbate + 2 H2O
-
 ?
1.10.3.3 chlorohydroquinone + O2 6% of activity with L-ascorbic acid Cucurbita sp. chloroquinone + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.3 55
-
 no loss in activity after 30 min, approx. 55% activity after 30 min at 60°C, approx. 30% activity after 30 min at 65°C Cucurbita sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.3 6.5
-
-
 Cucurbita sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.3 5 10 rapid loss of activity below pH 4 and above pH 12 Cucurbita sp.