Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Amon, A.; Markakis, P.
    Properties of ascorbate oxidase isozymes (1973), Phytochemistry, 12, 2127-2132.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.10.3.3 no change in total activity in the presence of 5 M urea, conversion of tetrameric form into monomer with 75% activity in 8 M urea Cucurbita pepo medullosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.3 Urea effect on various molecular forms Cucumis sativus
1.10.3.3 Urea effect on various molecular forms Cucurbita pepo condensa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.3 0.181
-
 L-ascorbate spectrophotometric method Cucumis sativus
1.10.3.3 0.2
-
 L-ascorbate spectrophotometric method Cucurbita pepo condensa
1.10.3.3 0.98
-
 L-ascorbate Warburg method Cucurbita pepo condensa
1.10.3.3 1.125
-
 L-ascorbate Warburg method Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.3 30000
-
 1 * 30000, enzyme also exists as dimer and tetramer, SDS-PAGE Cucumis sativus
1.10.3.3 30000
-
 2 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Cucumis sativus
1.10.3.3 30000
-
 4 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Cucumis sativus
1.10.3.3 35000
-
 1 * 35000, enzyme also exists as tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 35000
-
 4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 35000
-
 4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo medullosa
1.10.3.3 35000
-
 8 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 35000
-
 12 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 35000
-
 x * 35000, between 670000 Da and 2000000 Da, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.3 Cucumis sativus
-
 cucumber, 3 molecular forms: monomer, dimer, tetramer
-
1.10.3.3 Cucurbita pepo condensa
-
 5 molecular forms: monomer, tetramer, octamer, dodecamer, polymer
-
1.10.3.3 Cucurbita pepo medullosa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.3 fruit
-
 Cucumis sativus
-
1.10.3.3 fruit
-
 Cucurbita pepo condensa
-
1.10.3.3 fruit
-
 Cucurbita pepo medullosa
-
1.10.3.3 peel
-
 Cucumis sativus
-
1.10.3.3 peel
-
 Cucurbita pepo condensa
-
1.10.3.3 peel
-
 Cucurbita pepo medullosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.3 2 L-ascorbate + O2
-
 Cucumis sativus 2 L-dehydroascorbate + 2 H2O
-
 ?
1.10.3.3 2 L-ascorbate + O2
-
 Cucurbita pepo condensa 2 L-dehydroascorbate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.3 dimer 2 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Cucumis sativus
1.10.3.3 dodecamer 12 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 monomer 1 * 30000, enzyme also exists as dimer and tetramer, SDS-PAGE Cucumis sativus
1.10.3.3 monomer 1 * 35000, enzyme also exists as tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 octamer 8 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 polymer x * 35000, between 670000 Da and 2000000 Da, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 tetramer 4 * 30000, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Cucumis sativus
1.10.3.3 tetramer 4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo condensa
1.10.3.3 tetramer 4 * 35000, enzyme exists as monomer, tetramer, octamer, dodecamer and polymer, SDS-PAGE Cucurbita pepo medullosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.3 additional information
-
-
 Cucurbita pepo medullosa
1.10.3.3 additional information
-
 different molecular forms vary in resistance to heat inactivation: tetramer of squash and dimer of cucumber being most resistant Cucumis sativus
1.10.3.3 additional information
-
 different molecular forms vary in resistance to heat inactivation: tetramer of squash and dimer of cucumber being most resistant Cucurbita pepo condensa
1.10.3.3 40 50 conversion of octamer and heavier forms to a dimer Cucumis sativus
1.10.3.3 40 50 conversion of octamer and heavier forms to a dimer Cucurbita pepo condensa
1.10.3.3 100
-
 1 min, all forms of enzyme inactivated Cucumis sativus
1.10.3.3 100
-
 1 min, all forms of enzyme inactivated Cucurbita pepo condensa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.3 3.6
-
 loss of 80% activity after 30 min at 0°C Cucurbita pepo medullosa