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Literature summary extracted from

  • Lee, M.H.; Dawson, C.R.
    Ascorbate oxidase (1979), Methods Enzymol., 62, 30-39.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.3 azide
-
 Cucumis sativus
1.10.3.3 azide
-
 Cucurbita pepo condensa
1.10.3.3 azide competitive vs. ascorbate, noncompetitive vs. O2 Cucurbita pepo medullosa
1.10.3.3 F-
-
 Cucumis sativus
1.10.3.3 F-
-
 Cucurbita pepo condensa
1.10.3.3 F- competitive vs. ascorbate, noncompetitive vs. O2 Cucurbita pepo medullosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.3 copper
-
 Cucurbita pepo condensa
1.10.3.3 copper
-
 Cucurbita pepo medullosa
1.10.3.3 copper 10 copper atoms per enzyme molecule Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.3 70000
-
 2 * 70000, 70000 Da subunit consists of 2 polypeptide chains of 30000 and 40000 Da respectively Cucurbita pepo medullosa
1.10.3.3 140000
-
-
 Cucumis sativus
1.10.3.3 140000
-
 sedimentation equilibrium and difussion studies Cucurbita pepo medullosa

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.3 Cucumis sativus
-
-
-
1.10.3.3 Cucurbita pepo condensa
-
-
-
1.10.3.3 Cucurbita pepo medullosa
-
 green zucchini squash
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.3
-
 Cucurbita pepo condensa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.10.3.3 reconstitution of copper-free apoascorbate oxidase with cuprous copper solution Cucurbita pepo medullosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.3 fruit
-
 Cucumis sativus
-
1.10.3.3 fruit
-
 Cucurbita pepo condensa
-
1.10.3.3 fruit
-
 Cucurbita pepo medullosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.3 2 L-ascorbate + O2
-
 Cucumis sativus 2 L-dehydroascorbate + 2 H2O
-
 ?
1.10.3.3 2 L-ascorbate + O2
-
 Cucurbita pepo condensa 2 L-dehydroascorbate + 2 H2O
-
 ?
1.10.3.3 2 L-ascorbate + O2
-
 Cucurbita pepo medullosa 2 L-dehydroascorbate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.3 dimer
-
 Cucumis sativus
1.10.3.3 dimer 2 * 70000, 70000 Da subunit consists of 2 polypeptide chains of 30000 and 40000 Da respectively Cucurbita pepo medullosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.3 80
-
-
 Cucurbita pepo medullosa